| JaLCDOI |
10.18926/AMO/31831
|
| フルテキストURL |
fulltext.pdf
|
| 著者 |
Hatipoglu, Omer Faruk|
Hirohata, Satoshi|
Yaykasli, Kursat Oguz|
Cilek, Mehmet Zeynel|
Demircan, Kadir|
Shinohata, Ryoko|
Yonezawa, Tomoko|
Oohashi, Toshitaka|
Kusachi, Shozo|
Ninomiya, Yoshifumi|
|
| 抄録 |
<p>ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1) is an inflammatory-induced gene. We have previously reported that ADAMTS1 was strongly but transiently expressed in the infarcted heart. In this study, we investigated whether a 3'-untranslated region (UTR) affects the mRNA stability of this gene. When stimulated with tissue necrosis factor (TNF)-alpha, the expression level of ADAMTS1 mRNA rapidly increased, but the induction of ADAMTS1 mRNA peaked at 6h after stimulation, after which the expression levels of ADAMTS1 mRNA decreased. The 3'-UTR ADAMTS1 mRNA contains multiple adenine and uridine-rich elements, suggesting that the 3'-UTR may regulate gene stability. The addition of actinomycin D, an RNA synthesis inhibitor, demonstrated the decay of induced ADAMTS1 mRNA by TNF-alpha. Furthermore, a region containing multiple AUUUA motifs within the ADAMTS1 3'-UTR destabilized transfected Enhanced Green Fluorescence Protein (EGFP) mRNA expression. These results demonstrated that the ADAMTS1 3'-UTR may regulate the expression of ADAMTS1 mRNA.</p>
|
| キーワード |
ADAMTS1
gene regulation
metalloproteinase
|
| Amo Type |
Original Article
|
| 発行日 |
2009-04
|
| 出版物タイトル |
Acta Medica Okayama
|
| 巻 |
63巻
|
| 号 |
2号
|
| 出版者 |
Okayama University Medical School
|
| 開始ページ |
79
|
| 終了ページ |
85
|
| ISSN |
0386-300X
|
| NCID |
AA00508441
|
| 資料タイプ |
学術雑誌論文
|
| 言語 |
English
|
| 論文のバージョン |
publisher
|
| 査読 |
有り
|
| PubMed ID |
19404339
|
| Web of Science KeyUT |
000265457600002
|
