著者 城崎 由紀|
発行日 2005-03-25
出版物タイトル
資料タイプ 学位論文
JaLCDOI 10.18926/15363
フルテキストURL Mem_Fac_Eng_OU_34_39.pdf
著者 早川 聡| 城崎 由紀| 籔田 武司| 都留 寛治| Ohtsuki Chikara| 尾坂 明義|
抄録 Silicone elastomer was chemically treated at 60℃ for 7 days with 30 wt% H(2)O(2) solutions with or without TaCl(5) and soaked for various periods in a simulated body fluid(Kokubo solution) up to 21 days. Apatite formation ability of the surface of the silicone elastomer specimens was investigated with thin-film X-ray diffraction and FT-IR reflection spectroscopy. These silicone specimens did not deposit apatite or calcium phosphates, irrespective of chemical treatment. Osteoblast-like cells (MC3T3-El) derived from mouse were cultured on the specimens at 36.5℃ under 5%C0(2) and 95% humidity. Similar degree of proliferation of cells was observed at 7 days among three specimens, while the no treatment specimen after incubation for 5 days showed a lower degree of proliferation than the silicone treated with 30 wt% H(2)O(2) solutions with or without TaCl(5). Alkaline phosphatase activity of the cells proliferated on the no treatment specimen was lower than those of the silicone treated with 30 wt% H(2)O(2) solutions with or without TaCl(5). These results indicate that the cytotoxicity of the silicone could be improved by the chemical treatment with 30 wt% H(2)O(2) solutions with or without TaCl(5).
出版物タイトル Memoirs of the Faculty of Engineering, Okayama University
発行日 2000-03-27
34巻
1-2号
開始ページ 39
終了ページ 43
ISSN 0475-0071
言語 English
論文のバージョン publisher
NAID 120002308019
JaLCDOI 10.18926/47005
フルテキストURL mfe_36_1_117_121.pdf
著者 Shirosaki, Yuki| Tsuru, Kanji| Hayakawa, Satoshi| Osaka, Akiyoshi| Takashima, Seisuke|
抄録 γ-Methacryloxypropyltrimethoxysilane (γ-MPS) was grafted to silicone due to emulsion polymerization to induce Si-OH groups, in order to provide silicone with bioactivity spontaneous deposition of apatite in body fluid and to improve cytocompatibility. Apatite deposited on the grafted silicone within 7 days of soaking in 1.5 times as concentrated as the Kokubo solution. Osteoblastic cells (MC3T3-E1) were cultured on the specimens up to 7 days. After 5 days of culture, the number of MC3T3-E1 cells on the grafted specimen was much greater than that on the original specimen. These results indicated that the biocompatibility of silicone elastomer was improved by the grafting γ-MPS.
出版物タイトル Memoirs of the Faculty of Engineering, Okayama University
発行日 2001-12
36巻
1号
開始ページ 117
終了ページ 121
ISSN 0475-0071
言語 English
論文のバージョン publisher
NAID 120003497031