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ID 60133
フルテキストURL
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著者
Makanae, Aki Okayama University Research Core for Interdisciplinary Sciences (RCIS)
Tajika, Yuki Gunma University, Department of Anatomy, Graduate School of Medicine
Nishimura, Koki Okayama University Research Core for Interdisciplinary Sciences (RCIS)
Saito, Nanami Okayama University Research Core for Interdisciplinary Sciences (RCIS)
Tanaka, Jun-Ichi Okayama University Research Core for Interdisciplinary Sciences (RCIS)
Satoh, Akira Okayama University Research Core for Interdisciplinary Sciences (RCIS) Kaken ID
抄録
The presence of nerves is an important factor in successful organ regeneration in amphibians. The Mexican salamander, Ambystoma mexicanum, is able to regenerate limbs, tail, and gills when nerves are present. However, the nerve-dependency of tooth regeneration has not been evaluated. Here, we reevaluated tooth regeneration processes in axolotls using a three-dimensional reconstitution method called CoMBI and found that tooth regeneration is nerve-dependent although the dentary bone is independent of nerve presence. The induction and invagination of the dental lamina were delayed by denervation. Exogenous Fgf2, Fgf8, and Bmp7 expression could induce tooth placodes even in the denervated mandible. Our results suggest that the role of nerves is conserved and that Fgf+Bmp signals play key roles in axolotl organ-level regeneration. The presence of nerves is an important factor in successful organ regeneration in amphibians. The Mexican salamander, Ambystoma mexicanum, is able to regenerate limbs, tail, and gills when nerves are present. However, the nervedependency of tooth regeneration has not been evaluated. Here, we reevaluated tooth regeneration processes in axolotls using a three-dimensional reconstitution method called CoMBI and found that tooth regeneration is nerve-dependent although the dentary bone is independent of nerve presence. The induction and invagination of the dental lamina were delayed by denervation. Exogenous Fgf2, Fgf8, and Bmp7 expression could induce tooth placodes even in the denervated mandible. Our results suggest that the role of nerves is conserved and that Fgf+Bmp signals play key roles in axolotl organ-level regeneration.
キーワード
Cell proliferation
Differentiation
Morphogenesis
発行日
2020-06-09
出版物タイトル
Scientific Reports
10巻
1号
出版者
Nature Research
開始ページ
9323
ISSN
2045-2322
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
© The Author(s) 2020
論文のバージョン
publisher
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1038/s41598-020-66142-2
ライセンス
http://creativecommons.org/licenses/by/4.0/