start-ver=1.4
cd-journal=joma
no-vol=452
cd-vols=
no-issue=
article-no=
start-page=104
end-page=113
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190815
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Fgf- and Bmp-signaling regulate gill regeneration in Ambystoma mexicanum
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Gill regeneration has not been well studied compared to regeneration of other appendages, such as limb and tail regeneration. Here, we focused on axolotl gill regeneration and found that Fgf- and Bmp-signaling are involved in their gill regeneration mechanism. Axolotls have three pairs of gill rami, and each gill ramus has multiple gill filaments. The gills consist of mesenchyme rich in extracellular matrix and epidermis. The gill nerves are supplied from the trigeminal ganglia located in the head. Denervation resulted in no gill regeneration responses. Nerves and gills express Bmp and Fgf genes, and treating animals with Fgf- and Bmp-signaling inhibitors results in phenotypes similar to those seen in denervated gills. Inducing an accessory appendage is a standard assay in amphibian regeneration research. In our study, an accessory gill could be induced by lateral wounding, suggesting that thin axon fibers and mesenchymal Fgfs and Bmps contributed to the induction of the accessory structure. Such accessory gill induction was inhibited by the denervation. Exogenous Fgf2+Fgf8+Bmp7, which have been determined to function as a regeneration inducer in urodele amphibians, could compensate for the effects denervation has on accessory blastema formation. Our findings suggest that regeneration of appendages in axolotls is regulated by common Fgf- and Bmp-signaling cascades.
en-copyright=
kn-copyright=

en-aut-name=Saito Nanami
en-aut-sei=Saito
en-aut-mei= Nanami
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=Nishimura Koki
en-aut-sei=Nishimura
en-aut-mei= Koki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=Makanae Aki
en-aut-sei=Makanae
en-aut-mei= Aki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SatohAkira
en-aut-sei=Satoh
en-aut-mei=Akira
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Okayama University, Research Core for Interdisciplinary Sciences (RCIS)
kn-affil=

affil-num=2
en-affil=Okayama University, Research Core for Interdisciplinary Sciences (RCIS)
kn-affil=

affil-num=3
en-affil=Okayama University, Research Core for Interdisciplinary Sciences (RCIS)
kn-affil=

affil-num=4
en-affil=Okayama University, Research Core for Interdisciplinary Sciences (RCIS)
kn-affil=
en-keyword=Blastema induction
kn-keyword=Blastema induction
en-keyword= Bmp
kn-keyword= Bmp
en-keyword=Fgf
kn-keyword=Fgf
en-keyword=Gill regeneration
kn-keyword=Gill regeneration
en-keyword=Nerve
kn-keyword=Nerve
en-keyword=Organ regeneration
kn-keyword=Organ regeneration
END

start-ver=1.4
cd-journal=joma
no-vol=10
cd-vols=
no-issue=1
article-no=
start-page=9323
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200609
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Neural regulation in tooth regeneration of Ambystoma mexicanum
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The presence of nerves is an important factor in successful organ regeneration in amphibians. The Mexican salamander, Ambystoma mexicanum, is able to regenerate limbs, tail, and gills when nerves are present. However, the nerve-dependency of tooth regeneration has not been evaluated. Here, we reevaluated tooth regeneration processes in axolotls using a three-dimensional reconstitution method called CoMBI and found that tooth regeneration is nerve-dependent although the dentary bone is independent of nerve presence. The induction and invagination of the dental lamina were delayed by denervation. Exogenous Fgf2, Fgf8, and Bmp7 expression could induce tooth placodes even in the denervated mandible. Our results suggest that the role of nerves is conserved and that Fgf+Bmp signals play key roles in axolotl organ-level regeneration. The presence of nerves is an important factor in successful organ regeneration in amphibians. The Mexican salamander, Ambystoma mexicanum, is able to regenerate limbs, tail, and gills when nerves are present. However, the nervedependency of tooth regeneration has not been evaluated. Here, we reevaluated tooth regeneration processes in axolotls using a three-dimensional reconstitution method called CoMBI and found that tooth regeneration is nerve-dependent although the dentary bone is independent of nerve presence. The induction and invagination of the dental lamina were delayed by denervation. Exogenous Fgf2, Fgf8, and Bmp7 expression could induce tooth placodes even in the denervated mandible. Our results suggest that the role of nerves is conserved and that Fgf+Bmp signals play key roles in axolotl organ-level regeneration.
en-copyright=
kn-copyright=

en-aut-name=MakanaeAki
en-aut-sei=Makanae
en-aut-mei=Aki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TajikaYuki
en-aut-sei=Tajika
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NishimuraKoki
en-aut-sei=Nishimura
en-aut-mei=Koki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SaitoNanami
en-aut-sei=Saito
en-aut-mei=Nanami
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TanakaJun-Ichi
en-aut-sei=Tanaka
en-aut-mei=Jun-Ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=SatohAkira
en-aut-sei=Satoh
en-aut-mei=Akira
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Okayama University Research Core for Interdisciplinary Sciences (RCIS)
kn-affil=

affil-num=2
en-affil=Gunma University, Department of Anatomy, Graduate School of Medicine
kn-affil=

affil-num=3
en-affil=Okayama University Research Core for Interdisciplinary Sciences (RCIS)
kn-affil=

affil-num=4
en-affil=Okayama University Research Core for Interdisciplinary Sciences (RCIS)
kn-affil=

affil-num=5
en-affil=Okayama University Research Core for Interdisciplinary Sciences (RCIS)
kn-affil=

affil-num=6
en-affil=Okayama University Research Core for Interdisciplinary Sciences (RCIS)
kn-affil=
en-keyword=Cell proliferation
kn-keyword=Cell proliferation
en-keyword=Differentiation
kn-keyword=Differentiation
en-keyword=Morphogenesis
kn-keyword=Morphogenesis
END