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ID 58281
フルテキストURL
fulltext.pdf 15.9 MB
著者
Hassan, Ghmkin Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University ORCID
Afify, Said M. Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University ORCID
Nair, Neha Graduate School of Natural Science and Technology, Okayama University
Kumon, Kazuki Graduate School of Natural Science and Technology, Okayama University
Osman, Amira Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Du, Juan Graduate School of Natural Science and Technology, Okayama University
Mansour, Hager Graduate School of Natural Science and Technology, Okayama University
Abu Quora, Hagar A. Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Nawara, Hend M. Graduate School of Natural Science and Technology, Okayama University
Satoh, Ayano Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University ORCID Kaken ID publons researchmap
Zahra, Maram H. Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Okada, Nobuhiro Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Seno, Akimasa Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University ORCID Kaken ID researchmap
Seno, Masaharu Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University ORCID Kaken ID publons researchmap
抄録
Cancer stem cells (CSCs) represent the subpopulation of cancer cells with the ability to differentiate into other cell phenotypes and initiated tumorigenesis. Previously, we reported generating CSCs from mouse induced pluripotent stem cells (miPSCs). Here, we investigated the ability of the CSCs to differentiate into hematopoietic cells. First, the primary cells were isolated from malignant tumors that were formed by the CSCs. Non-adherent cells (NACs) that arose from adherent cells were collected and their viability, as well as the morphology and expression of hematopoietic cell markers, were analyzed. Moreover, NACs were injected into the tail vein of busulfan conditioned Balb/c nude mice. Finally, CSCs were induced to differentiate to macrophages while using IL3 and SCF. The round nucleated NACs were found to be viable, positive for hematopoietic lineage markers and CD34, and expressed hematopoietic markers, just like homing to the bone marrow. When NACs were injected into mice, Wright-Giemsa staining showed that the number of white blood cells got higher than those in the control mice after four weeks. CSCs also showed the ability to differentiate toward macrophages. CSCs were demonstrated to have the potential to provide progenies with hematopoietic markers, morphology, and homing ability to the bone marrow, which could give new insight into the tumor microenvironment according to the plasticity of CSCs.
キーワード
Induced pluripotent stem cells
Cancer stem cells differentiation
tumor microenvironment
hematopoietic cells
発行日
2019-12-29
出版物タイトル
Cancers
12巻
1号
出版者
MDPI
開始ページ
82
ISSN
2072-6694
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
© 2019 by the authors.
論文のバージョン
publisher
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.3390/cancers12010082
ライセンス
http://creativecommons.org/licenses/by/4.0/
助成機関名
文部科学省
助成番号
25242045
18K15243