このエントリーをはてなブックマークに追加
ID 30886
JaLCDOI
フルテキストURL
Thumnail fulltext.pdf 1.12 MB
著者
Akagi, Tadaatsu Okayama University
Takata, Hiroshi Okayama University
Yoshino, Tadashi Okayama University
Teramoto, Norihiro Okayama University
Yano, Shoki Kochi Medical School
Oka, Takashi Kochi Medical School
抄録

Co-cultivation of thymus and spleen cells of Fisher and Lewis rats with lethally irradiated MT-2 cells harboring human T-cell leukemia virus type I (HTLV-I) resulted in the establishment of lymphoid cell lines, FIRT-1, FIRS-1, LERT-1, and LERS-1, respectively. Cells of these cell lines had rat T-cell characters as demonstrated by the positive reaction to monoclonal antibodies (MAbs) to rat T cell antigens (Thy 1 and pan T). They lacked surface immunoglobulins and strongly expressed rat interleukin-2 receptor antigen (Tac) and Ia antigen. Karyotypic analysis revealed that they had the normal rat karyotype in early cultures, but showed marked aneuploidy after long cultivation. None of them expressed HTLV gag proteins (p19 and p24) or virus particles, but they contained HTLV-I proviral DNA monoclonally and weakly expressed pX gene products (p40x). They were not transplantable into syngeneic newborn rats.

キーワード
human T-cell leukemia virus
rat T cell
immortalization
Amo Type
Article
発行日
1989-06
出版物タイトル
Acta Medica Okayama
43巻
3号
出版者
Okayama University Medical School
開始ページ
143
終了ページ
151
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT