タイトル(別表記) A comparative gene analysis reveals a diatom-specific SET domain protein family
フルテキストURL srfa_110_001_006.pdf
著者 根本 理子|
抄録  The silica cell walls of diatoms, which exhibit species-spe-cific micro- and nano- patterned structures are promising candidates for applications in nanotechnology. Previous studies revealed a number of silica cell wall-associated proteins involved in silica formation. However, molecular biological analyses toward understanding of diatom cell wall formation have been mostly limited to model diatom species and general silica formation process in diatoms is still incompletely understood. In this study, to gain a compre-hensive insight into diatom silica biomineralization, tran-scriptome data of three diatom species, Nitzschia palea, Achnanthes kuwaitensis and Pseudoleyanella lunata, were newly developed. The reads obtained from RNA sequencing were assembled into 31,946, 60,767 and 38,314 unique transcripts for N. palea, A. kuwaitensis and P. lunata, respectively. In order to identify the diatom-specific genes, three transcriptome data sets developed in this study and the protein-coding gene sets of five genome-sequenced diatoms were compared. The proteins shared only by eight diatom species that are predicted to possess an endoplasmic reticulum (ER)-targeting signal peptide were selected for further analyses. These include proteins showing homology to silicanin-1, a recently reported diatom-specific protein involved in silica formation, as well as a number of SET domain proteins. The SET domain proteins might be novel diatom-specific family of methyltransferases that may reg-ulate the function of silica formation related proteins or long chain polyamines. The genes encoding the diatom-specific SET domain proteins identified in this study, which were shown to respond to silicon were suggested to be implicated in silica biomineralization.
キーワード Biomineralization Diatom Silica Protein
出版物タイトル 岡山大学農学部学術報告
発行日 2021-02-01
110巻
開始ページ 1
終了ページ 6
ISSN 2186-7755
言語 Japanese
論文のバージョン publisher
著者 Nemoto, Michiko| Iwaki, Sayako| Moriya, Hisao| Monden, Yuki| Tamura, Takashi| Inagaki, Kenji| Mayama, Shigeki| Obuse, Kiori|
キーワード Biomineralization Diatom Silica Transcriptome Proteome
備考 This is a post-peer-review, pre-copyedit version of an article published in Marine Biotechnology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10126-020-09976-1. </br>This fulltext is available in June 2021.|
発行日 2020-06-03
出版物タイトル Marine Biotechnology
出版者 Springer
ISSN 1436-2228
NCID AA11357643
資料タイプ 学術雑誌論文
言語 French
OAI-PMH Set 岡山大学
論文のバージョン author
PubMed ID 32488507
DOI 10.1007/s10126-020-09976-1
Web of Science KeyUT 000537432000001
関連URL isVersionOf https://doi.org/10.1007/s10126-020-09976-1
著者 Tamura, Takashi| Tsunekawa, Naoki| Nemoto, Michiko| Inagaki, Kenji| Hirano, Toshiyuki| Sato, Fumitoshi|
発行日 2016-01-28
出版物タイトル Scientific reports
6巻
資料タイプ 学術雑誌論文
著者 Oshima, Kenshiro| Hattori, Masahira| Shimizu, Hitomi| Fukuda, Koji| Nemoto, Michiko| Inagaki, Kenji| Tamura, Takashi|
発行日 2015-07-09
出版物タイトル Genome Announcements
3巻
4号
資料タイプ 学術雑誌論文