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ID 30724
JaLCDOI
フルテキストURL
著者
Fujita, Osamu Okayama University
Asanuma, Masato Okayama University
Yokoyama, Teruhiko Okayama University
Miyazaki, Ikuko Okayama University Kaken ID researchmap
Ogawa, Norio Okayama University
Kumon, Hiromi Okayama University
抄録
We examined the involvement of the signal transducer and activator of transcription 3 (STAT3) in bladder outlet obstruction (BOO)-induced bladder smooth muscle hypertrophy using a rat in vivo and in vitro study. BOO induced increases in bladder weight and bladder smooth muscle thickness 1 week after the operation. By using antibody microarrays, 64 of 389 proteins blotted on the array met our selection criteria of an INR value between > or = 2.0 and < or = 0.5. This result revealed up-regulation of transcription factors, cell cycle regulatory proteins, apoptosis-associated proteins and so on. On the other hand, down-regulation (INR value < or = 0.5) of proteins was not found. In a profiling study, we found an increase in the expression of STAT3. A significant increase in nuclear phosphorylated STAT3 expression was confirmed in bladder smooth muscle tissue by immunohistochemistry and Western blot analysis. Cyclical stretch-relaxation (1 Hz) at 120% elongation significantly increased the expression of STAT3 and of alpha-smooth muscle actin in primary cultured bladder smooth muscle cells. Furthermore, the blockade of STAT3 expression by the transfection of STAT3 small interfering RNA (siRNA) significantly prevented the stretch-induced increase in alpha-smooth muscle actin expression. These results suggest that STAT3 has an important role in the induction of bladder smooth muscle hypertrophy.
キーワード
benign prostatic hyperplasia
bladder outlet obstruction
bladder smooth muscle
signal transducer and activator of transcription 3 (STAT3)
small interfering RNA (siRNA)
Amo Type
Original Article
発行日
2006-12
出版物タイトル
Acta Medica Okayama
60巻
6号
出版者
Okayama University Medical School
開始ページ
299
終了ページ
309
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Science KeyUT