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ID 917
Eprint ID
917
フルテキストURL
タイトル(別表記)
傷害処理したカボチャ果肉組織から抽出したACC酸化酵素の2,3の性質
著者
マソホーコ フランシス・ムティーソ 岡山大学
久保 康隆 岡山大学
稲葉 昭次 岡山大学
中村 怜之輔 岡山大学
抄録
1-Aminocyclopropane-1-carboxylate (ACC) oxidase, the enzyme that catalyzes the conversion of ACC to ethylene, the final step of ethylene biosynthesis was extracted from wounded mesocarp tissue of winter squash (Cucurbita maxima Duch. v. Ebisu) fruit. The enzyme was characterized with respect to temperature optima, thermostability, stability in the presence f selected metal ions and alkylating agents, and Km value for ACC.   ACC oxidase requires Fe2+ as a co-factor and maximum activity was achieved using Fe2+ at 20uM in the reaction mixture. The enzyme was activated by C02 and inclusion of C02 in the reaction mixture increased the apparent Km value of the enzyme with respect to ACC. The enzyme exhibited apparent Km values for ACC of 147uM in air and 454 uM in the presence of 5 % C02. ACC oxidase was partially inactivated by ACC during its catalytic action. The enzyme had maximum activity at 30℃ and its activity was almost completely lost m the presence of Ag+, C02+, Cu2+ and Zn2+ ions. The alkylating agents, iodoacetamide and iodoacetic acid partially inhibited and almost completely abolished ACC oxidase activity respectively, thereby suggesting requirement of sulfhydryl groups for ACC oxidase activity. Following excision of the mesocarp tissue, the increase in in vivo ACC oxidase activity was accompanied by a concomitant increase in in vitro ACC oxidase activity.  The results support and extend previous observations and indicate that the solubilized enzyme is indeed ACC oxidase and resembles the physiological ACC oxidase in several aspects.
キーワード
カボチャ
果肉組織
ACC酸化酵素
性質
発行日
1993
出版物タイトル
岡山大学農学部学術報告
出版物タイトル(別表記)
Scientific Reports of the Faculty of Agriculture Okayama University
82巻
1号
出版者
岡山大学農学部
出版者(別表記)
Faculty of Agriculture, Okayama University
開始ページ
49
終了ページ
59
ISSN
0474-0254
NCID
AN00033029
資料タイプ
紀要論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
publisher
査読
無し
Eprints Journal Name
srfa