著者 Tetsunaga, Tomonori| Furumatsu, Takayuki| Abe, Nobuhiro| Nishida, Keiichiro| Naruse, Keiji| Ozaki, Toshifumi|
発行日 2009-09-18
出版物タイトル Journal of Biomechanics
42巻
13号
資料タイプ 学術雑誌論文
フルテキストURL fulltext.pdf
著者 Ohtsuki, Takashi| Shinaoka, Akira| Kumagishi-Shinaoka, Kanae| Asano, Keiichi| Hatipoglu, Omer Faruk| Inagaki, Junko| Takahashi, Ken| Oohashi, Toshitaka| Nishida, Keiichiro| Naruse, Keiji| Hirohata, Satoshi|
発行日 2019-10-15
出版物タイトル Experimental Cell Research
383巻
2号
出版者 Elsevier
開始ページ 111556
ISSN 00144827
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 © 2019 The Authors. Published by Elsevier Inc.
論文のバージョン publisher
PubMed ID 31415758
DOI 10.1016/j.yexcr.2019.111556
Web of Science KeyUT 000488145400017
関連URL isVersionOf https://doi.org/10.1016/j.yexcr.2019.111556
著者 Demircan, Kadir| Hirohata, Satoshi| Nishida, Keiichiro| Hatipoglu, Omer F.| Oohashi, Toshitaka| Yonezawa, Tomoko| Apte, Suneel S.| Ninomiya, Yoshifumi|
発行日 2005-5
出版物タイトル Arthritis & Rheumatism
52巻
5号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/30384
フルテキストURL fulltext.pdf
著者 Nishida, Keiichiro| Inoue, Hajime| Toda, Kazukiyo| Murakami, Takuro|
抄録 <p>Localization of the glycosaminoglycans (GAG) was examined in the synovial membranes of patients with osteoarthritis under light microscopy using a fine cationic colloidal iron staining method combined with enzymatic digestion. Our staining method was very useful for demonstrating the difference in the localization of GAG in regions of the inflammatory site in the osteoarthritic synovial membrane. Hyaluronic acid was mainly located in connective tissues in the surface intercellular and perivascular spaces, chondroitin sulfate A/C in the highly fibrous part of and connective tissue around blood vessels, dermatan sulfate (chondroitin sulfate B) in the subsurface interstitium and vascular endothelial cells and heparan sulfate in part of vascular endothelial cells. No keratan sulfate was detected. GAG is reported to have an important role in cell movement, adherence and aggregation in the inflammatory sites. These findings should be useful for understanding the role of GAG in physiological and pathologic processes of secondary synovitis.</p>
キーワード glycosaminoglycan synovial tissue osteoarthritis fine cationic colloidal iron
Amo Type Article
発行日 1995-12
出版物タイトル Acta Medica Okayama
49巻
6号
出版者 Okayama University Medical School
開始ページ 287
終了ページ 294
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8770237
Web of Science KeyUT A1995TM84600003
JaLCDOI 10.18926/AMO/30749
フルテキストURL fulltext.pdf
著者 Komiyama, Takamitsu| Nishida, Keiichiro| Yorimitsu, Masanori| Doi, Hideyuki| Miyazawa, Shinichi| Kitamura, Ai| Yoshida, Aki| Nasu, Yoshihisa| Abe, Nobuhiro| Ozaki, Toshifumi|
抄録 Ossification disturbance in femoral head reportedly is seen in the Spontaneously Hypertensive rats (SHR) between ages of 10 and 20 weeks. We investigated serum and tissue levels of insulin-like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) in SHR relevant to the ossification disturbance and osteonecrosis of the femoral head. Serum levels of IGF-1 and VEGF were significantly lower in SHR than in Wistar Kyoto rats (WKY) at weeks 5, 10, 15 and 20 (p&#60;0.005). The incidence of histological ossification disturbance of the femoral head was higher in SHR (59%) than in WKY (40%) at week 20. Lower serum and local levels of VEGF in SHR appeared to be related to the incomplete ossification of the femoral heads. Immunohistochemical study showed significantly lower numbers of IGF-1 and VEGF positive chondrocytes in the femoral epiphyseal cartilage of SHR than in those of WKY at weeks 10, 15 and 20. Our results suggest that local and/or systemic levels of IGF-1 and VEGF between ages of 5 and 20 weeks might play roles in the pathogenesis of ossifi cation disturbance of the femoral head in SHR.
キーワード spontaneous hypertensive rats insulin like growth factor-1 vascular endothelial growth factor ossification disturbance osteonecrosis
Amo Type Article
発行日 2006-06
出版物タイトル Acta Medica Okayama
60巻
3号
出版者 Okayama University Medical School
開始ページ 141
終了ページ 148
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 16838042
Web of Science KeyUT 000238503600001
JaLCDOI 10.18926/AMO/30753
フルテキストURL fulltext.pdf
著者 Nakatani, Satoru| Naito, Ichiro| Momota, Ryusuke| Hinenoya, Noriko| Horiuchi, Kanji| Nishida, Keiichiro| Ohtsuka, Aiji|
抄録 <p>We attempted to prepare colloidal iron within tissues by means of microwave irradiation. Mouse tissue blocks were fixed with a mixture of paraformaldehyde and ferric chloride in a cacodylate buffer, immersed in a cacodylate buffered ferric chloride solution, and irradiated in a microwave processor. Colloidal iron was prepared within tissues or cells, and was observed in the form of electron dense fine granules (1-2 nm in diameter) by transmission electron microscopy. Collagen fibrils in the connective tissue showed colloidal iron deposition at regular periodical intervals. Cells in the splenic tissue showed that fine colloidal granules were deposited on the ribosomes but not on the nuclear chromatin. This finding suggests that ferric ions could not diffuse into the nucleus, which was surrounded by the nuclear envelope. The podocyte processes of the renal glomerulus were stained diffusedly. Though this microwave in situ colloidal iron preparation method has some limitations, it is convenient for use in biomedical specimen preparation in transmission electron microscopy.</p>
キーワード colloidal iron microwave histochemistry transmission electron microscopy
Amo Type Article
発行日 2006-02
出版物タイトル Acta Medica Okayama
60巻
1号
出版者 Okayama University Medical School
開始ページ 59
終了ページ 64
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 16508690
Web of Science KeyUT 000235538900007
JaLCDOI 10.18926/AMO/30778
フルテキストURL fulltext.pdf
著者 Kinugasa, Kiyoto| Hashizume, Hiroyuki| Nishida, Keiichiro| Shigeyama, Yukio| Inoue, Hajime|
抄録 <p>The results of the histological examinations of specimens of the tenosynovium of the flexor tendon, the epineurium and the transverse carpal ligament from two groups of Japanese patients with carpal tunnel syndrome (idiopathic and hemodialysis) were compared. Amyloid deposits, positively identified as &#946;<sub>2</sub>-microglobulin, appeared in all patients in the long-term hemodialysis group, but in no patients in the idiopathic group. Although the pathogenesis differed between the two groups, both resulted in nerve compression in the carpal tunnel. Therefore, surgical release is considered beneficial for both groups.</p>
キーワード carpal tunnel syndrome histopathology clinical results idiopathic hemodialysis
Amo Type Article
発行日 1997-04
出版物タイトル Acta Medica Okayama
51巻
2号
出版者 Okayama University Medical School
開始ページ 63
終了ページ 70
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9142342
Web of Science KeyUT A1997WX19600002
JaLCDOI 10.18926/AMO/30790
フルテキストURL fulltext.pdf
著者 Nanba, Yoshifumi| Nishida, Keiichiro| Yoshikawa, Takeshi| Sato, Toru| Inoue, Hajime| Kuboki, Yoshinori|
抄録 <p>The expression of osteonectin (ON) in osteoarthritic articular cartilage was investigated by enzyme immunohistochemistry and colloidal gold immunoelectron microscopy. A total of 96 specimens from 9 knees of 8 patients with osteoarthritis (OA) were examined. In OA cartilage, ON-positive cells varied in distribution and were not seen in all the specimens obtained from the same patient. However, in over half of the specimens (56 of 96), especially in the specimens of Mankin's grades from 4 to 9, which corresponds to relatively early stages of OA, ON was expressed in the cartilage above the calcified layer. On the other hand, ON was detected only in the calcified layer below the tidemark in normal articular cartilage. In addition, colloidal gold immunoelectron microscopy revealed ON in chondrocytes and matrix vesicles (MVs). These findings suggest that ON acts through MVs in the early stages of OA as a significant pathogenetic factor involved in intracartilage calcification, which is known to have a close relationship to the progression of OA.</p>
キーワード chondrocalcinosis osteoarthritis osteonection Mankin's histologic-histochemical grading calcification
Amo Type Article
発行日 1997-10
出版物タイトル Acta Medica Okayama
51巻
5号
出版者 Okayama University Medical School
開始ページ 239
終了ページ 243
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9359920
Web of Science KeyUT A1997YD65300001
JaLCDOI 10.18926/AMO/30794
フルテキストURL fulltext.pdf
著者 Nagoshi, Mitsuru| Hashizume, Hiroyuki| Nishida, Keiichiro| Takagoshi, Hidekazu| Pu, Jean| Inoue, Hajime|
抄録 <p>Sixty-seven trigger fingers of 58 idiopathic and hemodialysis patients were treated by percutaneous A1-pulley release technique. Severity of triggering was classified into five grades for treatment selection and prediction of possible results. Results were excellent in 41 fingers, good in 9, fair in 7, and poor in 10, requiring additional treatment. The results of the lower grades were better, and those of the higher grades were poor. Excellent or good results appeared to depend on the proper selection of the patients according to the grading system and confirmation of triggering disappearance just after the release. There were neither infections nor neuro-vascular deficits after treatment. Compared to conventional open release, this treatment was found to be more useful from the standpoints of ease and safety of the technique, and the patients' quick return to normal life.</p>
キーワード trigger finger percutaneous release idiopathic hemodialysis
Amo Type Article
発行日 1997-06
出版物タイトル Acta Medica Okayama
51巻
3号
出版者 Okayama University Medical School
開始ページ 155
終了ページ 158
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9227795
Web of Science KeyUT A1997XJ12700007
JaLCDOI 10.18926/AMO/30974
フルテキストURL fulltext.pdf
著者 Ikuma, Hisanori| Abe, Nobuhiro| Uchida, Youichiro| Furumatsu, Takayuki| Fujiwara, Kazuo| Nishida, Keiichiro| Ozaki, Toshifumi|
抄録 <p>Instability of the knee after the medial collateral ligament (MCL) injury is usually assessed with the manual valgus stress test, even though, in recent years, it has become possible to apply magnetic resonance imaging (MRI) to the assessment of the damage of the ligament. The valgus instability of 24 patients (12 isolated injuries and 12 multiple ligament injuries) who suffered MCL injury between 1993 and 1998 was evaluated with the Hughston and Eilers classification, which involves radiographic assessment under manual valgus stress to the injured knees. We developed a novel system for classifying the degree of injury to the MCL by calculating the percentage of injured area based on MRI and investigated the relationship between this novel MRI classification and the magnitude of valgus instability by the Hughston and Eilers classification. There was a significant correlation between the 2 classifications (p=0.0006). On the other hand, the results using other MRI based classification systems, such as the Mink and Deutsch classificaiton and the Petermann classification, were not correlated with the findings by the Hughston and Eilers classification in these cases (p0.05). Since MRI is capable of assessing the injured ligament in clinical practice, this novel classification system would be useful for evaluating the stability of the knee and choosing an appropriate treatment following MCL injury.</p>
キーワード medial collateral ligament magnetic resonance imaging knee instability novel method
Amo Type Original Article
発行日 2008-06
出版物タイトル Acta Medica Okayama
62巻
3号
出版者 Okayama University Medical School
開始ページ 185
終了ページ 191
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 18596835
Web of Science KeyUT 000257130300006
JaLCDOI 10.18926/AMO/31338
フルテキストURL fulltext.pdf
著者 Takagoshi, Hidekazu| Hashizume, Hiroyuki| Nishida, Keiichiro| Masaoka, shunji| Asahara, Hiroshi| Inoue, Hajime|
抄録 <p>The fibrous components of the metacarpophalangeal (MP) joint including the palmar plate, the collateral ligament and the dorsal plate were studied with particular attention paid to the fibrous structure of the fibrous tendon sheath and the deep transverse metacarpal ligament. The tough fibrillar structure around the MP joint, especially the force nucleus, consisted of three types of mixed fibers: the fibrous tendon sheath of the A1 pulley, the deep transverse metacarpal ligament, and the palmar plate. The tendon sheath was located on the ulnar side in the index and middle fingers, on the central position in the ring finger, and on the radial side in the little finger. These fibrous connections among the fingers formed a transverse arch in the hand. The palmar plate of the MP joint was relatively rigid and appears to function as a cushion when flexed. A fold-like protrusion of the synovial layer of the palmar plate of the MP joint had a meniscoid function, which was larger than that of the proximal interphalangeal joint. The capsule of the MP joint was thicker at the dorsal area, forming a dorsal plate, which is a sliding floor of the extensor mechanism and has a meniscoid function for joint congruity. The main lateral stabilizer consisted of collateral ligaments and accessory collateral ligaments anchored to the palmar plate. These structures act together as a &#34;phalangeal cuff&#34;, connecting the proximal phalanx to the metacarpal head and sta</p>
キーワード metacarpophalangeal joint collagen framework
Amo Type Article
発行日 1998-02
出版物タイトル Acta Medica Okayama
52巻
1号
出版者 Okayama University Medical School
開始ページ 19
終了ページ 26
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9548990
Web of Science KeyUT 000072264100003
JaLCDOI 10.18926/AMO/31339
フルテキストURL fulltext.pdf
著者 Pu, Jian| Nishida, Keiichiro| Inoue, Hajime| Asahara, Hiroshi| Ohtsuka, Aiji| Murakami, Takuro|
抄録 <p>Mast cells in osteoarthritic and rheumatoid arthritic synovial tissues of the human knee.</p>
キーワード mast cell count osteoaethritis rheumatoid arthritis hydroarthrosis synovial membrance
Amo Type Article
発行日 1998-02
出版物タイトル Acta Medica Okayama
52巻
1号
出版者 Okayama University Medical School
開始ページ 35
終了ページ 39
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9548992
Web of Science KeyUT 000072264100005
JaLCDOI 10.18926/AMO/31711
フルテキストURL fulltext.pdf
著者 Kosaka, Motohiro| Horiuchi, Kanji| Nishida, Keiichiro| Taguchi, Takehito| Murakami, Takuro| Ohtsuka, Aiji|
抄録 <p>The celiac and mesenteric arterial system including the left gastric, splenic, common hepatic, and superior mesenteric arteries shows various types of origins, courses, ramifications and anastomoses. In order to explain the various expressions of this system, we have proposed a typological model, in which celiacomesenteric arteries develop as paired or bilaterally symmetrical primordial vessels originated from the anterior aspect of the aorta, and these vessels anastomose each other with longitudinal and horizontal pathways. Here, we report 3 unusual cases characterized by arterial rings, formed by the left gastric, left accessory hepatic, proper hepatic, anterior pancreaticoduodenal, and dorsal pancreatic arteries. The dorsal pancreatic and anterior pancreaticoduodenal arteries are located to the right and left of the embryonic pancreas developing in the dorsal mesentery, respectively. Such hepatopancreatic arterial rings simultaneously containing right and left elements can only be explained using our typological model, in which the concept of paired arteries or bilateral symmetry is introduced.</p>
キーワード arterial variation celiac trunk superior mesenteric artery typology bilateral symmetry
Amo Type Article
発行日 2002-10
出版物タイトル Acta Medica Okayama
56巻
5号
出版者 Okayama University Medical School
開始ページ 245
終了ページ 253
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 12530508
Web of Science KeyUT 000178668100005
JaLCDOI 10.18926/AMO/31820
フルテキストURL fulltext.pdf
著者 Miyake, Kohei| Nishida, Keiichiro| Kadota, Yasutaka| Yamasaki, Hiroko| Nasu, Tatsuyo| Saitou, Daisuke| Tanabe, Katsuyuki| Sonoda, Hikaru| Sato, Yasufumi| Maeshima, Yohei| Makino, Hirofumi|
抄録 <p>Angiogenesis is an essential event in the development of synovial inflammation in rheumatoid arthritis (RA). The aim of the current study was to investigate the expression of vasohibin-1, a novel endothelium-derived vascular endothelial growth factor (VEGF)-inducible angiogenesis inhibitor, in the RA synovium, and to test the effect of inflammatory cytokines on the expression of vasohibin-1 by RA synovial fibroblasts (RASFs). Synovial tissue samples were obtained at surgery from patients with osteoarthritis (OA) and RA, and subjected to immunohistochemistry to investigate the expression and distribution of vasohibin-1 relevant to the degree of synovial inflammation. In an in vitro analysis, RASFs were used to examine the expression of vasohibin-1 and VEGF mRNA by real-time PCR after stimulation with VEGF or inflammatory cytokines under normoxic or hypoxic conditions. The immunohistochemical results showed that vasohibin-1 was expressed in synovial lining cells, endothelial cells, and synovial fibroblasts. In synovial tissue, there was a significant correlation between the expression of vasohibin-1 and histological inflammation score (p0.002, r0.842). In vitro, stimulation with VEGF induced the expression of vasohibin-1 mRNA in RASFs under normoxic conditions, and stimulation with cytokines induced vasohibin-1 mRNA expression under a hypoxic condition. These results suggest that vasohibin-1 was expressed in RA synovial tissue and might be regulated by inflammatory cytokines.</p>
キーワード angiogenesis vasohibin-1 rheumatoid arthritis synovial membrane VEGF
Amo Type Original Article
発行日 2009-12
出版物タイトル Acta Medica Okayama
63巻
6号
出版者 Okayama University Medical School
開始ページ 349
終了ページ 358
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 20035291
Web of Science KeyUT 000273145900006
JaLCDOI 10.18926/AMO/31823
フルテキストURL fulltext.pdf
著者 Tanaka, Masato| Nakanishi, Kazuo| Sugimoto, Yoshihisa| Misawa, Haruo| Takigawa, Tomoyuki| Nishida, Keiichiro| Ozaki, Toshifumi|
抄録 <p>Scoliosis is a common clinical manifestation of Rett syndrome, a neurodevelopmental disorder that almost exclusively affects females. The spinal curve in patients with Rett syndrome is typically a long C curve of a neuromuscular type. As the onset of the scoliosis is very early and shows rapid progression, early surgical intervention has been recommended to prevent a life-threatening collapsing spine syndrome. However, there are high perioperative risks in Rett syndrome patients who undergo spinal surgery, such as neurological compromise and respiratory dysfunction due to rigid spinal curve. We herein report the surgical result of treating severe rapid progressive thoracic scoliosis in a 16-year-old girl with Rett syndrome. Posterior segmental pedicle screw fixation was performed from T1 to L3 using a computer-assisted technique. Post-operative radiography demonstrated a good correction of the curve in both the sagittal and coronal alignment. There were no postoperative complications such as neurological compromise. The patient had maintained satisfactory spinal balance as of the 3-year follow-up examination.</p>
キーワード Rett syndrome scoliosis computer navigation-assisted surgery segmental pedicle screw fixation
Amo Type Case Report
発行日 2009-12
出版物タイトル Acta Medica Okayama
63巻
6号
出版者 Okayama University Medical School
開始ページ 373
終了ページ 377
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 20035294
Web of Science KeyUT 000273145900009
JaLCDOI 10.18926/AMO/32000
フルテキストURL fulltext.pdf
著者 Matsuo, Masatsugu| Nishida, Keiichiro| Yoshida, Aki| Murakami, Takuro| Inoue, Hajime|
抄録 <p>To clarify the involvement of the caspase family in the pathway of NO-induced chondrocyte apoptosis, osteoarthritis (OA) cartilage obtained from 8 patients undergoing total hip arthroplasty were used for histopathological study. Cartilage samples taken from non-fibrillated areas of femoral head resected during surgery for femoral neck fracture were used for comparison. DNA fragmentation of chondrocytes was detected by the nick end-labeling (TUNEL) method. Apoptosis was further confirmed by transmission electron microscopy. The distributions of nitrotyrosine (NT), caspase-3, and -9 were examined immunohistochemically. The populations of apoptotic as well as NT-, caspase-3-, and -9-positive cells were quantified by counting the number of cells in the superficial, middle, and deep layers, respectively. The TUNEL-positive cells were observed primarily in superficial proliferating chondrocytes, clustering chondrocytes, and deep-layer chondrocytes of OA cartilage. Few positive cells were seen in the proliferating chondrocytes in the middle layer. Positive reactions for caspase-3 and -9 were observed in chondrocytes in similar areas. Histological OA grade showed significant correlations with the mean populations of apoptotic chondrocytes (% apoptosis) over the 3 areas. The populations of NT-positive cells (% NT) over the same areas also showed significant correlation with OA grade. Positivity for caspase-3 closely correlated with the OA grade, % apoptosis and %NT. It was concluded that caspase-3 and -9 could play a role in NO-induced chondrocyte apoptosis in OA cartilage.</p>
キーワード apoptosis caspase nitric oxide osteoarthritis chondrocyte
Amo Type Article
発行日 2001-12
出版物タイトル Acta Medica Okayama
55巻
6号
出版者 Okayama University Medical School
開始ページ 333
終了ページ 340
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 11779095
Web of Science KeyUT 000172838400003
JaLCDOI 10.18926/AMO/32287
フルテキストURL fulltext.pdf
著者 Shibahara, Motomi| Nishida, Keiichiro| Asahara, Hiroshi| Yoshikawa, Takeshi| Mitani, Shigeru| Kondo, Yoichi| Inoue, Hajime|
抄録 <p>We investigated the presence of osteocyte apoptosis in the necrotic trabeculae of the femoral head of spontaneously hypertensive rat (SHR) using the in situ nick end labeling (TUNEL) method and transmission electron microscopy. The occurrence of osteonecrosis and ossification disturbance was significantly higher in SHR compared with Wistar Kyoto (WKY) rats, and Wistar (WT) rats used as control animals (P < 0.01). A high population of TUNEL positive osteocytes was detected mainly in 10- and 15-week-old SHRs. Sectioned examination of the femoral head of SHRs and WKY rats by electron microscopy revealed apoptotic cell appearances such as aggregation of chromatin particles and lipid formation. In contrast, a positive reaction was significantly lower in osteocytes in the femoral heads of WT rats (P < 0.01). Our results indicate that apoptosis forms an important component of the global pathologic process affecting the femoral head of SHR, which leads to osteonecrosis in this region.</p>
キーワード apoptosis spontaneously hypertensive rat osteonecrosis of the femoral head
Amo Type Article
発行日 2000-04
出版物タイトル Acta Medica Okayama
54巻
2号
出版者 Okayama University Medical School
開始ページ 67
終了ページ 74
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 10806527
Web of Science KeyUT 000086735900003
JaLCDOI 10.18926/AMO/47262
フルテキストURL 65_6_369.pdf
著者 Terada, Chuji| Yoshida, Aki| Nasu, Yoshihisa| Mori, Shuji| Tomono, Yasuko| Tanaka, Masato| Takahashi, Hideo K.| Nishibori, Masahiro| Ozaki, Toshifumi| Nishida, Keiichiro|
抄録 We investigated the expression and localization of high-mobility group box chromosomal protein-1 (HMGB-1) in human osteoarthritic (OA) cartilage in relation to the histopathological grade of cartilage destruction, and examined the role of HMGB-1 in the regulation of proinflammatory cytokine expression in chondrocytes. An immunohistochemical study demonstrated that total HMGB-1-positive cell ratios increase as the Osteoarthritis Research Society International (OARSI) histological grade increased. The population of cytoplasmic HMGB-1-positive chondrocytes was especially increased in the deep layers of higher-grade cartilage. The ratios and localization of receptors for advanced glycation end products (RAGE) expression by chondrocytes in Grade 2, 3, and 4 were significantly higher than those in Grade 1. In vitro stimulation with IL-1β, but not TNFα, significantly upregulated the expression of HMGB-1 mRNA by human OA chondrocytes. Both IL-1β and TNFα promoted the translocation of HMGB-1 from nuclei to cytoplasm. IL-1β and TNFα secretions were stimulated at higher levels of HMGB-1. The results of our study suggest the involvement of HMGB-1 in the pathogenesis of cartilage destruction in OA.
キーワード HMGB-1 RAGE chondrocyte osteoarthritis cartilage
Amo Type Original Article
発行日 2011-12
出版物タイトル Acta Medica Okayama
65巻
6号
出版者 Okayama University Medical School
開始ページ 369
終了ページ 377
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2011 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 22189477
Web of Science KeyUT 000298516900003
JaLCDOI 10.18926/AMO/49255
フルテキストURL 67_1_35.pdf
著者 Watanabe, Masutaka| Arita, Seizaburo| Hashizume, Hiroyuki| Honda, Mitsugi| Nishida, Keiichiro| Ozaki, Toshifumi|
抄録 The purpose of this study was to quantitatively evaluate Akahori's preoperative classification of cubital tunnel syndrome. We analyzed the results for 57 elbows that were treated by a simple decompression procedure from 1997 to 2004. The relationship between each item of Akahori's preoperative classification and clinical stage was investigated based on the parameter distribution. We evaluated Akahori's classification system using multiple regression analysis, and investigated the association between the stage and treatment results. The usefulness of the regression equation was evaluated by analysis of variance of the expected and observed scores. In the parameter distribution, each item of Akahori's classification was mostly associated with the stage, but it was difficult to judge the severity of palsy. In the mathematical evaluation, the most effective item in determining the stage was sensory conduction velocity. It was demonstrated that the established regression equation was highly reliable (R=0.922). Akahori's preoperative classification can also be used in postoperative classification, and this classification was correlated with postoperative prognosis. Our results indicate that Akahori's preoperative classification is a suitable system. It is reliable, reproducible and well-correlated with the postoperative prognosis. In addition, the established prediction formula is useful to reduce the diagnostic complexity of Akahori's classification.
キーワード cubital tunnel syndrome ulnar nerve Akahoriʼs classification multiple regression analysis
Amo Type Original Article
発行日 2013-02
出版物タイトル Acta Medica Okayama
67巻
1号
出版者 Okayama University Medical School
開始ページ 35
終了ページ 44
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2013 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 23439507
Web of Science KeyUT 000316829900005
関連URL http://ousar.lib.okayama-u.ac.jp/metadata/50647
JaLCDOI 10.18926/AMO/53119
フルテキストURL 69_1_29.pdf
著者 Nakahara, Ryuichi| Nishida, Keiichiro| Hashizume, Kenzo| Harada, Ryouzou| Machida, Takahiro| Horita, Masahiro| Ohtsuka, Aiji| Ozaki, Toshifumi|
抄録 The outcome measures in rheumatology clinical trials (OMERACT) scores are the most mature quantitation system for rheumatoid arthritis (RA) on magnetic resonance imaging (MRI). Direct measuring techniques of synovial volume have been reported with good reproducibility, although few reports have demonstrated the changes of these measures in response to treatment. To assess these clinical responses, we evaluated the correlation of the changes of clinical activity score 28-joints disease activity score (DAS28-CRP) with the changes of OMERACT scores and with synovial volume measurements. Eight RA patients who were treated by biologic agents were examined with MRI of the dominant affected wrist and finger joints before and one year after the treatment. The total OMERACT score was reduced from 48.0 to 41.3, and synovial volume was reduced from 15.4 to 8.8 milliliters. Positive correlations were seen between the changes of DAS28-CRP and the changes of OMERACT synovitis score (r=0.27), OMERACT total score (r=0.43) and synovial volume (r=0.30). Limited to synovium assessment, synovial volume showed a better correlation with DAS28-CRP than the OMERACT synovitis score. On the other hand, the OMERACT total score showed a higher correlation with DAS28-CRP than synovial volume, probably because the OMERACT total score includes scores for bone erosion and bone edema as well.
キーワード magnetic resonance imaging rheumatoid arthritis outcome measures in rheumatology clinical trials scoring system direct volume measuring medical work station
Amo Type Original Article
発行日 2015-02
出版物タイトル Acta Medica Okayama
69巻
1号
出版者 Okayama University Medical School
開始ページ 29
終了ページ 35
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2015 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 25703168
Web of Science KeyUT 000349740300003
関連URL http://ousar.lib.okayama-u.ac.jp/metadata/53113