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ID 32188
JaLCDOI
フルテキストURL
著者
Zhang, Bo Okayama University
Watanabe, Sekiko Okayama University
Akiyama, Kosuke Okayama University
Li, Ting Okayama University
Fukushima, Keisuke Okayama University
Tsutsui, Ken Okayama University
Seki, Shuji Okayama University
抄録

DNA repair synthesis induced in permeable mouse ascites sarcoma cells by peplomycin, an antitumor antibiotic, was studied. Mouse ascites sarcoma (SR-C3H/He) cells were permeabilized with a low concentration of Triton X-100 in an isotonic condition. Permeable cells were treated with an appropriate concentration of peplomycin to introduce single-strand breaks in permeable cell DNA. DNA repair synthesis in peplomycin-treated permeable cells was measured by incubating the cells with four deoxynucleoside triphosphates in an appropriate buffer system. The DNA repair synthesis was enhanced by ATP and NaCl at near physiological concentrations. More than 90% of DNA synthesis in the present system depended on the peplomycin-treatment. The repair nature of the DNA synthesis was confirmed by a BrdUMP density shift technique. The repair patches were largely completed and ligated in the presence of ATP. Analyses using selective inhibitors for DNA polymerases showed that both DNA polymerase Beta and aphidicolin-sensitive DNA polymerases (DNA polymerase alpha and/or delta) were involved in the repair DNA synthesis.</P>

キーワード
DNA repair
peplomycin
DNA polymerases
permeable mouse cells
Amo Type
Article
発行日
1991-04
出版物タイトル
Acta Medica Okayama
45巻
2号
出版者
Okayama University Medical School
開始ページ
89
終了ページ
94
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT