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ID 30889
JaLCDOI
フルテキストURL
著者
Sumii, Hiroshi Okayama University
Tsutsui, Ken Okayama University
Hatsushika, Masao Okayama University
Inoue, Hajime Okayama University
Tanabe, Gozo Okayama University
oda, Takuzo Okayama University
抄録

Preparations of IgG2b purified from several mouse hybridoma clones were highly susceptible, compared to other subclasses, to peptic digestion under conditions usually used to prepare F (ab')2 fragments. Analyses of the digestion products revealed that no F (ab')2 was produced and that the main product was a Fab-like fragment. Demonstration of the hinge disulfides in the Fc portion clearly indicated that in IgG2b the primary peptic cleavage occurs on the NH2-terminal side of the inter-heavy chain disulfide bridge. The resulting Fab failed to bind with antigen, suggesting the importance of the CH1-hinge region in maintaining the native conformation of the antigen-binding site.

キーワード
monoclonal antibody
immunoglobulin G2b
f (ab')2
peptic digestion
maleimide compound
Amo Type
Article
発行日
1989-06
出版物タイトル
Acta Medica Okayama
43巻
3号
出版者
Okayama University Medical School
開始ページ
135
終了ページ
141
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT