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ID 17681
Eprint ID
17681
フルテキストURL
Thumnail 88_977.pdf 2.62 MB
タイトル(別表記)
Studies on the structure of nuclei and chromatin using low molecular weight polyanions
著者
筒井 研 岡山大学医学部癌源研究施設生化学部門
抄録
Synthetic dye, aluminon has been demonstrated to cause swelling of isolated nuclei, decondensation of chromatin fibers, and dissociation of histones from chromatin. These phenomena are similar to those caused by high molecular weight polyanions such as heparin. In this paper, nuclear swelling with aluminon was studied in more detail in order to establish the role of aluminon as a probe for nuclei and chromatin. Studies were also done on the binding of aluminon to nuclei, and on its effect on RNA synthesis in vitro. Nuclear swelling was monitored from changes in turbidity of nuclear suspension. A stoichiometrical relationship was suggested between the amount of aluminon needed for maximal swelling and that of nuclear DNA in the system. The swelling velocity at pH 5.4 was much slower than that at pH 7.9 and the time course of the swelling was shown to be consisted of four distinct successive processes in which the swelling velocity was constant. Centrifugation of treated chromatin in a linear density gradient of sucrose revealed a decreased density of chromatin and dissociation of histones and certain nonhistone proteins from chromatin. Aggregation of arginine rich histones after dissociation was observed. The release of histones from chromation was also deduced from the fact that aluminon removed the restriction of chromatin template when transcribed with homologous RNA polymerases. Above results indicate that aluminon may be a useful tool for studies on structure and function of chromatin.
発行日
1976-12-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
88巻
11-12号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
977
終了ページ
995
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/88/11-12/88_11-12_977/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma