著者 Shinaoka, Akira| Momota, Ryusuke| Shiratsuchi, Eri| Kosaka, Mitsuko| Kumagishi, Kanae| Nakahara, Ryuichi| Naito, Ichiro| Ohtsuka, Aiji|
発行日 2013-04
出版物タイトル Microscopy and Microanalysis
19巻
2号
資料タイプ 学術雑誌論文
著者 Momota, Ryusuke| Naito, Ichiro| Ninomiya, Yoshifumi| Ohtsuka, Aiji|
発行日 2011-05
出版物タイトル Matrix Biology
30巻
4号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/32859
フルテキストURL fulltext.pdf
著者 Murakami, Shinichiro| Fujino, Hidemi| Takeda, Isao| Momota, Ryusuke| Kumagishi, Kanae| Ohtsuka, Aiji|
抄録 <p>The skeletal muscle is classified into 2 types, slow oxidative or fast glycolytic muscle. For further characterization, we investigated the capillary architecture in slow and fast muscles. The rat soleus and extensor digitorum longus (EDL) muscles were used as representatives of slow and fast muscles, respectively. To investigate capillary density, sections of both types of muscle were stained with alkaline phosphatase;the soleus muscle showed more intense reactivity, indicating that it had a denser capillary structure than the EDL muscle. We then injected fluorescent contrast medium into samples of both muscle types for light and confocal-laser microscopic evaluation. The capillary density and capillary-to-fiber ratio were significantly higher, and the course of the capillaries was more tortuous, in the soleus muscle than in the EDL muscle. Capillary coursed more tortuously in the soleus than in the EDL muscle. Succinate dehydrogenase (SDH) activity, an indicator of mitochondrial oxidative capacity, and vascular endothelial growth factor (VEGF) expression were also significantly higher in the soleus muscle. Thus, we conclude that slow oxidative muscle possess a rich capillary structure to provide demanded oxygen, and VEGF might be involved in the formation and/or maintenance of this highly capillarized architecture.</p>
キーワード skeletal muscle capillaly succinate dehydrogenase activity vascular endothelial growth factor
Amo Type Original Article
発行日 2010-02
出版物タイトル Acta Medica Okayama
64巻
1号
出版者 Okayama University Medical School
開始ページ 11
終了ページ 18
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
論文のバージョン publisher
査読 有り
Submission Path amo/vol64/iss1/2
PubMed ID 20200579
Web of Sience KeyUT 000274868300002
JaLCDOI 10.18926/AMO/30753
フルテキストURL fulltext.pdf
著者 Nakatani, Satoru| Naito, Ichiro| Momota, Ryusuke| Hinenoya, Noriko| Horiuchi, Kanji| Nishida, Keiichiro| Ohtsuka, Aiji|
抄録 <p>We attempted to prepare colloidal iron within tissues by means of microwave irradiation. Mouse tissue blocks were fixed with a mixture of paraformaldehyde and ferric chloride in a cacodylate buffer, immersed in a cacodylate buffered ferric chloride solution, and irradiated in a microwave processor. Colloidal iron was prepared within tissues or cells, and was observed in the form of electron dense fine granules (1-2 nm in diameter) by transmission electron microscopy. Collagen fibrils in the connective tissue showed colloidal iron deposition at regular periodical intervals. Cells in the splenic tissue showed that fine colloidal granules were deposited on the ribosomes but not on the nuclear chromatin. This finding suggests that ferric ions could not diffuse into the nucleus, which was surrounded by the nuclear envelope. The podocyte processes of the renal glomerulus were stained diffusedly. Though this microwave in situ colloidal iron preparation method has some limitations, it is convenient for use in biomedical specimen preparation in transmission electron microscopy.</p>
キーワード colloidal iron microwave histochemistry transmission electron microscopy
Amo Type Article
発行日 2006-02
出版物タイトル Acta Medica Okayama
60巻
1号
出版者 Okayama University Medical School
開始ページ 59
終了ページ 64
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
論文のバージョン publisher
査読 有り
Submission Path amo/vol60/iss1/7
PubMed ID 16508690
Web of Sience KeyUT 000235538900007
著者 百田 龍輔|
発行日 2001-03-25
出版物タイトル
資料タイプ 学位論文