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ID 53008
フルテキストURL
著者
Murakami, Shinki Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Brain Sci
Miyazaki, Ikuko Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Brain Sci Kaken ID researchmap
Sogawa, Norio Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Dent Pharmacol
Miyoshi, Ko Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Brain Sci
Asanuma, Masato Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Brain Sci
抄録
Parkinson's disease (PD) is a neurodegenerative disease with motor symptoms as well as non-motor symptoms that precede the onset of motor symptoms. Mitochondrial complex I inhibitor, rotenone, has been widely used to reproduce PD pathology in the central nervous system (CNS) and enteric nervous system (ENS). We reported previously that metallothioneins (MTs) released from astrocytes can protect dopaminergic neurons against oxidative stress. The present study examined the changes in MT expression by chronic systemic rotenone administration in the striatum and colonic myenteric plexus of C57BL mice. In addition, we investigated the effects of MT depletion on rotenone-induced neurodegeneration in CNS and ENS using MT-1 and MT-2 knockout (MT KO) mice, or using primary cultured neurons from MT KO mice. In normal C57BL mice, subcutaneous administration of rotenone for 6 weeks caused neurodegeneration, increased MT expression with astrocytes activation in the striatum and myenteric plexus. MT KO mice showed more severe myenteric neuronal damage by rotenone administration after 4 weeks than wild-type mice, accompanied by reduced astroglial activation. In primary cultured mesencephalic neurons from MT KO mice, rotenone exposure induced neurotoxicity in dopaminergic neurons, which was complemented by addition of recombinant protein. The present results suggest that MT seems to provide protection against neurodegeneration in ENS of rotenone-induced PD model mice.
キーワード
Parkinson's disease
Rotenone
Enteric nervous system
Astrocytes
Metallothionein
発行日
2014-10
出版物タイトル
Neurotoxicity Research
26巻
3号
出版者
Springer
開始ページ
285
終了ページ
298
ISSN
1029-8428
資料タイプ
学術雑誌論文
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/52964
言語
English
論文のバージョン
author
査読
有り
DOI
Web of Science KeyUT