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ID 48566
JaLCDOI
フルテキストURL
著者
Sasaki, Takanori Departments of Radiology, Okayama University Graduate School of Medicine
Kuroda, Masahiro Radiological Technology, Graduate School of Health Sciences, Okayama University ORCID Kaken ID publons researchmap
Katashima, Kazunori Departments of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine
Ashida, Masakazu Departments of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine
Matsuzaki, Hidenobu Departments of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine Kaken ID
Asaumi, Junichi Departments of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine Kaken ID publons researchmap
Murakami, Jun Departments of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine
Ohno, Seiichiro Central Division of Radiology, Okayama University Hospital
Kato, Hirokazu Radiological Technology, Graduate School of Health Sciences, Okayama University
Kanazawa, Susumu Departments of Radiology, Okayama University Graduate School of Medicine Kaken ID publons
抄録
The roles of cell density, extracellular space, intracellular factors, and apoptosis induced by the molecularly targeted drug rituximab on the apparent diffusion coefficient (ADC) values were investigated using bio-phantoms. In these bio-phantoms, Ramos cells (a human Burkittセs lymphoma cell line) were encapsulated in gellan gum. The ADC values decreased linearly with the increase in cell density, and declined steeply when the extracellular space became less than 4 μm. The analysis of ADC values after destruction of the cellular membrane by sonication indicated that approximately 65% of the ADC values of normal cells originate from the cell structures made of membranes and that the remaining 35% originate from intracellular components. Microparticles, defined as particles smaller than the normal cells, increased in number after rituximab treatments, migrated to the extracellular space and significantly decreased the ADC values of bio-phantoms during apoptosis. An in vitro study using bio-phantoms was conducted to quantitatively clarify the roles of cellular factors and of extracellular space in determining the ADC values yielded by tumor cells and the mechanism by which apoptosis changes those values.
キーワード
apparent diffusion coefficient value
cell density
extracellular space
bio-phantom
Amo Type
Original Article
発行日
2012-06
出版物タイトル
Acta Medica Okayama
66巻
3号
出版者
Okayama University Medical School
開始ページ
263
終了ページ
270
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
著作権者
CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Science KeyUT