JaLCDOI 10.18926/AMO/53335
フルテキストURL 69_2_71.pdf
著者 Hiramoto, Hiroki| Dansako, Hiromichi| Takeda, Midori| Satoh, Shinya| Wakita, Takaji| Ikeda, Masanori| Kato, Nobuyuki|
抄録 Persistent infection with hepatitis C virus (HCV) often causes chronic hepatitis, and then shows a high rate of progression to liver cirrhosis and hepatocellular carcinoma. To clarify the mechanism of the persistent HCV infection is considered to be important for the discovery of new target(s) for the development of anti-HCV strategies. In the present study, we found that the expression level of annexin A1 (ANXA1) in human hepatoma cell line Li23-derived D7 cells was remarkably lower than that in parental Li23 cells, whereas the susceptibility of D7 cells to HCV infection was much higher than that of Li23 cells. Therefore, we hypothesized that ANXA1 negatively regulates persistent HCV infection through the inhibition of viral RNA replication. The results revealed that HCV production was significantly inhibited without a concomitant reduction in the amount of lipid droplets in the D7 cells stably expressing exogenous ANXA1. Further, we demonstrated that ANXA1 negatively regulated the step of viral RNA replication rather than that of viral entry in human hepatocytes. These results suggest that ANXA1 would be a novel target for the development of anti-HCV strategies.
キーワード HCV annexin A1 Li23 cell line Li23-derived D7 cells HCV-JFH-1
Amo Type Original Article
発行日 2015-04
出版物タイトル Acta Medica Okayama
出版者 Okayama University Medical School
開始ページ 71
終了ページ 78
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2015 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 25899628
Web of Science KeyUT 000353181700001
著者 有海 康雄| 黒木 美沙緒| 團迫 浩方| 阿部 健一| 池田 正徳| 脇田 隆字| 加藤 宣之|
発行日 2010-04-01
出版物タイトル 岡山医学会雑誌
資料タイプ 学術雑誌論文
著者 Kuroki, Misao| Ariumi, Yasuo| Hijikata, Makoto| Ikeda, Masanori| Dansako, Hiromichi| Wakita, Takaji| Shimotohno, Kunitada| Kato, Nobuyuki|
発行日 2013-01-11
出版物タイトル Biochemical and Biophysical Research Communications
資料タイプ 学術雑誌論文
著者 Dansako, Hiromichi| Ueda, Youki| Okumura, Nobuaki| Satoh, Shinya| Sugiyama, Masaya| Mizokami, Masashi| Ikeda, Masanori| Kato, Nobuyuki|
発行日 2015
出版物タイトル The FEBS journal
資料タイプ 学術雑誌論文
著者 Ueda, Youki| Takeda, Midori| Mori, Kyoko| Dansako, Hiromichi| Wakita, Takaji| Kim, Hye-Sook| Sato, Akira| Wataya, Yusuke| Ikeda, Masanori| Kato, Nobuyuki|
発行日 2013-08-30
出版物タイトル PLOS ONE
資料タイプ 学術雑誌論文
著者 Kato, Nobuyuki| Sejima, Hiroe| Ueda, Youki| Mori, Kyoko| Satoh, Shinya| Dansako, Hiromichi| Ikeda, Masanori|
発行日 2014-03-13
出版物タイトル PLOS ONE
資料タイプ 学術雑誌論文