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ID 30764
JaLCDOI
フルテキストURL
著者
Ino, Hideo Okayama University
Hayashi, Kazuhiko Okayama University
Yanai, Hiroyuki Okayama University
Teramoto, Norihiro Okayama University
Koirala, Tirtha Raj Okayama University
Chen, Hong-Li Okayama University
Oka, Takashi Okayama University Kaken ID publons researchmap
Yoshino, Tadashi Okayama University Kaken ID publons researchmap
Takahashi, Kiyoshi Okayama University
Akagi, Tadaastu Okayama University
抄録

A simian cell line, Si-IIA, harboring Epstein-Barr-virus (EBV) -related herpesvirus (Si-IIA-EBV), produces malignant lymphoma in rabbits when administered by intravenous inoculation. In this study, we analyzed the Si-IIA-EBV genome and compared it with human EBV and herpesvirus macaca fascicularis 1 (HVMF 1 ), which is associated with B-cell lymphoma developing in SIV-infected immunosuppressed monkeys. DNA from Si-IIA-EBV was amplified by the polymerase chain reaction using three different primer pairs complementary to human EBV (B95-8) DNA; two of the primer pairs covered part of the long internal repeat 1 region (IR 1) and the third covered part of the BRRF 1 region. Direct sequencing of the three PCR products revealed that Si-IIA-EBV DNA had about 82% nucleotide homology to the human EBV DNA in all three regions and 92.4% homology to HVMF1 in the IR1 region. The blotting pattern by Southern blot analysis was different between Si-IIA-EBV and human EBV.

キーワード
Epstein-Barr virus
HVMF 1
lymphoma
?monkey cell line
PCR
Amo Type
Article
発行日
1997-08
出版物タイトル
Acta Medica Okayama
51巻
4号
出版者
Okayama University Medical School
開始ページ
207
終了ページ
212
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Science KeyUT