著者 田辺 俊介| 白川 靖博| 前田 直見| 大原 利章| 野間 和広| 櫻間 教文| 柳井 広之| 山辻 知樹| 猶本 良夫| 藤原 俊義|
発行日 2012-08-01
出版物タイトル 岡山医学会雑誌
124巻
2号
資料タイプ 学術雑誌論文
著者 佐々木 剛| 田澤 大| 長谷井 嬢| 国定 俊之| 吉田 晶| 橋本 悠里| 矢野 修也| 吉田 亮介| 宇野 太| 香川 俊輔| 森本 裕樹| 浦田 泰生| 藤原 俊義| 尾﨑 敏文|
発行日 2012-08-01
出版物タイトル 岡山医学会雑誌
124巻
2号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/48268
フルテキストURL 66_2_177.pdf.pdf
著者 Utsumi, Masashi| Matsuda, Hiroaki| Sadamori, Hiroshi| Shinoura, Susumu| Umeda, Yuzo| Yoshida, Ryuichi| Satoh, Daisuke| Hashimoto, Masaaki| Yagi, Takahito| Fujiwara, Toshiyoshi|
抄録 We report 4 cases of surgical resection of metachronous lymph node (LN) metastases from hepatocellular carcinoma (HCC) following hepatectomy. Clinicopathological features and results of LN dissection were investigated in the 4 patients. One patient was found to have a single metastasis in the mediastinal LNs, another had multiple metastases in the mediastinal and abdominal LNs, and the other 2 had single metastases in the abdominal LN. The locations of the abdominal LN metastases were behind the pancreas head in 2 patients and around the abdominal aorta in 1 patient. They all underwent surgical resection of metastatic LNs and had no postoperative complications. The 3 patients whose LN metastases were solitary have been alive for more than 2 years after LN resection, and one of them is free from recurrence. The patient with multiple LN metastases died 13 months after LN resection due to carcinomatosis. With the expectation of long-term survival, a single metachronous LN metastasis from HCC after hepatectomy should be resected in patients without uncontrollable intrahepatic or extrahepatic tumors.
キーワード hepatocellular carcinoma lymph node metastasis hepatectomy
Amo Type Case Report
発行日 2012-04
出版物タイトル Acta Medica Okayama
66巻
2号
出版者 Okayama University Medical School
開始ページ 177
終了ページ 182
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 22525476
Web of Science KeyUT 000303175300011
JaLCDOI 10.18926/AMO/48258
フルテキストURL 66_2_83.pdf.pdf
著者 Kuroda, Shinji| Urata, Yasuo| Fujiwara, Toshiyoshi|
抄録 Radiotherapy plays a central part in cancer treatment, and use of radiosensitizing agents can greatly enhance this modality. Although studies have shown that several chemotherapeutic agents have the potential to increase the radiosensitivity of tumor cells, investigators have also studied a number of molecularly targeted agents as radiosensitizers in clinical trials based on reasonably promising preclinical data. Recent intense research into the DNA damage-signaling pathway revealed that ataxia-telangiectasia mutated (ATM) and the Mre11-Rad50-NBS1 (MRN) complex play central roles in DNA repair and cell cycle checkpoints and that these molecules are promising targets for radiosensitization. Researchers recently developed three ATM inhibitors (KU-55933, CGK733, and CP466722) and an MRN complex inhibitor (mirin) and showed that they have great potential as radiosensitizers of tumors in preclinical studies. Additionally, we showed that a telomerase-dependent oncolytic adenovirus that we developed (OBP-301 [telomelysin]) produces profound radiosensitizing effects by inhibiting the MRN complex via the adenoviral E1B55kDa protein. A recent Phase I trial in the United States determined that telomelysin was safe and well tolerated in humans, and this agent is about to be tested in combination with radiotherapy in a clinical trial based on intriguing preclinical data demonstrating that telomelysin and ionizing radiation can potentiate each other. In this review, we highlight the great potential of ATM and MRN complex inhibitors, including telomelysin, as radiosensitizing agents.
キーワード ATM (ataxia-telangiectasia mutated) MRN (Mre11-Rad50-NBS1) complex radiosensitization adenovirus E1B55kDa
Amo Type Review
発行日 2012-04
出版物タイトル Acta Medica Okayama
66巻
2号
出版者 Okayama University Medical School
開始ページ 83
終了ページ 92
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 22525466
Web of Science KeyUT 000303175300001
著者 西崎 正彦| 藤原 康宏| 丁田 泰宏| 金澤 卓| 二宮 基樹| 藤原 俊義|
発行日 2012-04-01
出版物タイトル 岡山医学会雑誌
124巻
1号
資料タイプ 学術雑誌論文
著者 篠浦 先| 八木 孝仁| 貞森 裕| 松田 浩明| 楳田 祐三| 吉田 龍一| 佐藤 太佑| 内海 方嗣| 横道 直佑| 杭瀬 崇| 藤原 俊義|
発行日 2012-04-01
出版物タイトル 岡山医学会雑誌
124巻
1号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/47265
フルテキストURL 65_6_395.pdf
著者 Harada, Sosuke| Sato, Shuhei| Suzuki, Etsuji| Okumura, Yoshihiro| Hiraki, Takao| Gobara, Hideo| Mimura, Hidefumi| Kanazawa, Susumu| Kaji, Mitsumasa| Fujiwara, Toshiyoshi|
抄録 The aim of the present study was to assess the diagnostic usefulness of Fluorine-18 fluorodeoxyglucose (18F-FDG) positron emission tomography/computed tomography (PET/CT) in the prediction of local recurrence of malignant lung tumors by analyzing the pre-radiofrequency ablation (RFA) maximal standardized uptake value (SUVmax). We performed a historical cohort study of consecutive malignant lung tumors treated by RFA from January 2007 to May 2008 at Okayama University Hospital. We selected only lung tumors examined by PET/CT within 90 days before RFA and divided them (10 primary and 29 metastatic) into 3 groups according to their tertiles of SUVmax. We calculated recurrence odds ratios in the medium group and the high group compared to the low group using multivariate logistic analysis. After we examined the relationship between SUVmax and recurrence in a crude model, we adjusted for some factors. Tumors with higher SUVmax showed higher recurrence odds ratios (medium group;1.84, high group;4.14, respectively). The tumor size also increased the recurrence odds ratio (2.67);we thought this was mainly due to selection bias because we excluded tumors less than 10mm in diameter. This study demonstrated the pre-RFA SUVmax in PET/CT may be a prognostic factor for local recurrence of malignant lung tumors.
キーワード fluorodeoxyglucose (FDG) positron emission tomography (PET) standardized uptake value (SUV) radiofrequency ablation (RFA) lung
Amo Type Original Article
発行日 2011-12
出版物タイトル Acta Medica Okayama
65巻
6号
出版者 Okayama University Medical School
開始ページ 395
終了ページ 402
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2011 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 22189480
Web of Science KeyUT 000298516900006
著者 佐藤 太祐| 八木 孝仁| 貞森 裕| 楳田 祐三| 藤原 俊義|
発行日 2011-12-01
出版物タイトル 岡山医学会雑誌
123巻
3号
資料タイプ 学術雑誌論文
著者 佐藤 太祐| 八木 孝仁| 貞森 裕| 松田 浩明| 篠浦 先| 楳田 祐三| 吉田 龍一| 内海 方嗣| 藤原 俊義|
発行日 2011-12-01
出版物タイトル 岡山医学会雑誌
123巻
3号
資料タイプ 学術雑誌論文
著者 黒田 新士| 藤原 俊哉| 白川 靖博| 山崎 泰源| 矢野 修也| 宇野 太| 田澤 大| 橋本 悠里| 渡辺 雄一| 野間 和広| 浦田 泰生| 香川 俊輔| 藤原 俊義|
発行日 2011-08-01
出版物タイトル 岡山医学会雑誌
123巻
2号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/46628
フルテキストURL 65_3_169.pdf
著者 Takeda, Masanori| Nagasaka, Takeshi| Dong-Sheng, Sun| Nishie, Hiroyuki| Oka, Tetsuhiro| Yamada, Eiji| Mori, Yoshiko| Shigeyasu, Kunitoshi| Morikawa, Tatsuya| Mizobuchi, Satoshi| Fujiwara, Toshiyoshi|
抄録 Secreted frizzled-related protein 2, (SFRP2) is a Wnt inhibitor whose promoter CpGs were recently found to be methylated at high frequency in colorectal cancers (CRCs). We hypothesized that the pattern of SFRP2 methylation may differ throughout the promoter during progressive tumorigenesis. Using combined bisulfite restriction analysis (COBRA), two methylation-sensitive regions (Regions A and B) of the SFRP2 promoter were investigated in 569 specimens of colorectal tissue:222 CRCs, 103 adenomatous polyps (APs), 208 normal colonic mucosa from CRC patients (N-Cs), and 36 normal colonic mucosa from subjects with no evidence of colorectal neoplasia at colonoscopy (N-Ns). Extensive (including both Regions A and B) and partial (either Region A or B) SFRP2 methylation levels were found in 61.7% and 24.8% of CRCs, 8.7% and 37.9% of APs, 3.9% and 39.9% of N-Cs, and 0% and 30.6% of N-Ns, respectively. Extensive methylation of the SFRP2 promoter was present primarily in CRCs, while partial methylation was common in APs. Whereas APs with the KRAS mutant showed no correlation to any pattern of SFRP2 methylation, extensive methylation of the SFRP2 promoter was significantly associated with KRAS mutant CRCs (p<.0001), suggesting that genetic alteration in the RAS-RAF pathway might precede the spread of CpG methylation through the SFRP2 promoter, which is observed in over 60% of advanced colorectal tumors.
キーワード BRAF/KRAS mutations promoter methylation colorectal cancer
Amo Type Original Article
発行日 2011-06
出版物タイトル Acta Medica Okayama
65巻
3号
出版者 Okayama University Medical School
開始ページ 169
終了ページ 177
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2011 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 21709714
Web of Science KeyUT 000292017500003
JaLCDOI 10.18926/AMO/46626
フルテキストURL 65_3_151.pdf
著者 Fujiwara, Toshiyoshi|
抄録 Replication-selective tumor-specific viruses constitute a novel approach for treatment of neoplastic disease. These vectors are designed to induce virus-mediated lysis of tumor cells after selective viral propagation within the tumor. Human telomerase is highly active in more than 85オ of primary cancers, regardless of their tissue origins, and its activity correlates closely with human telomerase reverse transcriptase (hTERT) expression. We constructed an attenuated adenovirus 5 vector (Telomelysin, OBP-301), in which the hTERT promoter element drives expression of E1 genes. Since only tumor cells that express telomerase activity would activate this promoter, the hTERT proximal promoter would allow for preferential expression of viral genes in tumor cells, leading to selective viral replication and oncolytic cell death. Lymphatic invasion is a major route for cancer cell dissemination, and adequate treatment of locoregional lymph nodes is required for curative treatment in patients with gastrointestinal tumors. We demonstrated that intratumoral injection of Telomelysin mediates effective in vivo purging of metastatic tumor cells from regional lymph nodes. Moreover, using noninvasive whole-body imaging, we found that intratumoral injection of Telomelysin followed by regional irradiation induces a substantial antitumor effect, resulting from tumor cell-specific radiosensitization, in an orthotopic human esophageal cancer xenograft model. These results illustrate the potential of oncolytic virotherapy as a promising strategy in the management of human gastrointestinal cancer.
キーワード telomerase adenovirus metastasis lymph node colorectal cancer
Amo Type Review
発行日 2011-06
出版物タイトル Acta Medica Okayama
65巻
3号
出版者 Okayama University Medical School
開始ページ 151
終了ページ 162
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2011 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 21709712
Web of Science KeyUT 000292017500001
著者 野間 和広| 田中屋 宏爾| 竹内 仁司| 小長 英二| 藤原 俊義|
発行日 2011-04-01
出版物タイトル 岡山医学会雑誌
123巻
1号
資料タイプ 学術雑誌論文
著者 藤原 俊義|
発行日 2010-12-01
出版物タイトル 岡山医学会雑誌
122巻
3号
資料タイプ その他
著者 藤原 俊義|
発行日 2010-12-01
出版物タイトル 岡山医学会雑誌
122巻
3号
資料タイプ 学術雑誌論文
著者 児島 亨| 橋本 悠里| 香川 俊輔| 田中 紀章| 浦田 泰生| 藤原 俊義|
発行日 2010-12-01
出版物タイトル 岡山医学会雑誌
122巻
3号
資料タイプ 学術雑誌論文
著者 永坂 岳司| 田中 紀章| 孫 冬生| 猶本 良夫| 松原 長秀| 八木 孝仁| 藤原 俊義|
発行日 2010-08-02
出版物タイトル 岡山医学会雑誌
122巻
2号
資料タイプ 学術雑誌論文
著者 Teraishi, Fuminori| Kagawa, Shunsuke| Watanabe, Takanori| Tango, Yasuhisa| Kawashima, Takeshi| Umeoka, Tatsuo| Nisizaki, Masahiko| Tanaka, Noriaki| Fujiwara, Toshiyoshi|
発行日 2005-08-01
出版物タイトル FEBS Letters
579巻
19号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/31570
フルテキストURL fulltext.pdf
著者 Matsuoka, Junji| Sakagami, Kenichi| Fujiwara, Toshiyoshi| Onoda, Tadashi| Idani, Hitoshi| Gochi, Akira| Orita, Kunzo|
抄録 <p>A sustained release system for interleukin-2 (IL-2), and IL-2 mini-pellet (IL-2 mp), was developed by fusing IL-2 into a needle shaped collagen. Serum concentration of IL-2 after a single subcutaneous injection of the IL-2 mp into C57BL/6 mice remained elevated longer than after an injection of aqueous IL-2. IL-2 in the serum became undetectable by 6h after a subcutaneous injection of 1 x 10(6) unit of IL-2 in phosphate-buffered saline (PBS). In contrast, after a single subcutaneous injection of IL-2 mp containing the same amount of IL-2, the concentration of IL-2 increased to its maximum at 6h after injection, then began to decrease gradually. IL-2 was detected even on the third day after a single subcutaneous injection of one IL-2 mp. Augmentation of NK activity and generation of IL-2 activated killer cells were observed in the spleen from day 1--day 3 after a single subcutaneous injection of IL-2 mp into C57BL/6 mice. This activation was not observed following a single subcutaneous injection of the same amount of IL-2 in PBS. Adoptive immunotherapy by a single subcutaneous injection of IL-2 mp followed by intravenous injections of in vitro cultured IL-2 activated killer cells showed better results in decreasing the number of metastases of Lewis lung carcinoma in C57BL/6 mice than immunotherapy using IL-2 solution.(ABSTRACT TRUNCATED AT 250 WORDS)</p>
キーワード IL-2 drug delivery system immunotherapy mouse
Amo Type Article
発行日 1993-04
出版物タイトル Acta Medica Okayama
47巻
2号
出版者 Okayama University Medical School
開始ページ 79
終了ページ 84
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8506753
Web of Science KeyUT A1993LA45200002
著者 Umeoka, Tatsuo| Kawashima, Takeshi| Kagawa, Shunsuke| Teraishi, Fuminori| Taki, Masaki| Nishizaki, Masahiko| Kyo, Satoru| Nagai, Katsuyuki| Urata, Yasuo| Tanaka, Noriaki| Fujiwara, Toshiyoshi|
発行日 2004-09-01
出版物タイトル Cancer Research
64巻
17号
資料タイプ 学術雑誌論文