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ID 60360
フルテキストURL
fulltext.pdf 2.46 MB
著者
Morikawa, Yuko Department of Pediatric Dentistry, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Morimoto, Setsuyo Department of Pediatric Dentistry, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Yoshida, Eri Department of Pediatric Dentistry, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Naka, Shuhei Department of Pediatric Dentistry, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Inaba, Hiroaki Department of Pediatric Dentistry, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Matsumoto-Nakano, Michiyo Department of Pediatric Dentistry, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kaken ID
抄録
Background
Streptococcus mutans, a biofilm-forming bacterium, possesses several transporters that function as import/export molecules. Among them, the PII protein family is composed of members that regulate glutamine synthesis in bacterial species.
Objective
In this study, we characterized the function of the glutamine transporter in S. mutans MT8148.
Methods
The SMU.732 gene, corresponding to glnP in S. mutans, is homologous to the glutamine transporter gene in Bacillus subtilis. We constructed a glnP-inactivated mutant strain (GEMR) and a complement strain (comp-GEMR) and evaluated their biological functions.
Results
Growth of GEMR was similar in the presence and absence of glutamine, whereas the growth rates of MT8148 and comp-GEMR were significantly lower in the presence of glutamine as compared to its absence. Furthermore, biofilms formed by MT8148 and comp-GEMR were significantly thicker than that formed by GEMR, while the GEMR strain showed a significantly lower survival rate in an acidic environment than the other strains. Addition of n-phenyl-2-naphthylamine, used to label of the membrane, led to increased fluorescence intensity of MT8148 and GEMR, albeit that was significantly lower in the latter.
Conclusions
These results suggest that glnP is associated with glutamine transport in S. mutans, especially the import of glutamine involved in biofilm formation.
キーワード
Streptococcus mutans
glutamine transporter
biofilm
membrane protein
glnP
発行日
2020-08-04
出版物タイトル
Journal of Oral Microbiology
12巻
1号
出版者
Taylor & Francis
開始ページ
1797320
ISSN
2000-2297
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
© 2020 The Author(s).
論文のバージョン
publisher
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1080/20002297.2020.1797320
ライセンス
https://creativecommons.org/licenses/by/4.0/
助成機関名
日本学術振興会
助成番号
20H03897
19K19294