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ID 40253
タイトル(別表記)
Studies on Paper-analysis in the Field of Balneology. (3) The Changes of the Serum Protein Fractions and Lipoprotein Fractions Following the Bathing in Radioactive Hot Spring
著者
石橋 丸応 岡山大学温泉研究所
抄録
I. Some fundamental conditions and procedures on filter-paper electrophoresis were investigated. The electrophoretic apparatus of Natsume Seisakusho and Toyo No. 51 filter-paper were used. Electrophoresis was carried out under the current of 0.25 mA/cm. in 300 Volt during 5 hours, using veronal buffer pH 8.6 (μ=0.05). In measuring of serum protein fractions, the adequate volume of the materials loaded is in the range of 0.005~0.01 ml. and in the case of lipoprotein, 0.02 ml. of serum is used. Paper-strip was stained with bromophenol blue or sudan black B, then making it transparent with heat-solved paraffin and photometered by Nataume's densitometer. The reproducibility of the above-mentioned method with a confidence of 99% was as follows: Serum protein fractions Albumin 53.4±1.8% α(1)-Globulin 3.3±0.3 α(2)-Globulin 6.0±0.5 β-Globulin 12.2±0.7 γ-Globulin 25.1±1.1 Serum lipoprotein fractions α 20.6±2.6% β+γ 79.4±2.6 II. Changes in serum protein fractions and lipoprotein fractions following the radioactive hot spring bathing were as follows: 1). In rabbits, the albumin-fraction of serum protein increased after a series of baths in radioactive hot spring, and the β- and γ-globulin-fractions decreased. 2). By cholesterol-feeding, the albumin-fraction of serum protein of rabbits decreased and the β- and γ-globulin-fractions increased significantly but when a series of baths was carried out in cholesterol-fed rabbits the decrease in the albumin-fraction was slighter than the former. 3). α-globulin-fraction of serum lipoprotein increased after single bathing in radioactive hot spring and (β+γ) -globulin-fractions showed an inverse change, but the changes of lipoprotein-fractions following a series of baths were not remarkable. 4). By cholesterol-feeding, the ratio of lipoprotein-fractions (β+γ/α) increased, but the ratio tended to decrease following a series of baths in radioactive hot spring.
備考
正誤表あり
発行日
1959-01
出版物タイトル
岡山大学温泉研究所報告
出版物タイトル(別表記)
Papers of the Institute for Thermal Spring Research, Okayama University
24巻
出版者
岡山大学温泉研究所
出版者(別表記)
Institute for Thermal Spring Research, Okayama University
開始ページ
54
終了ページ
79
ISSN
0369-7142
NCID
AN00032853
資料タイプ
紀要論文
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/40250
言語
Japanese
論文のバージョン
publisher
査読
無し
Eprints Journal Name
mmc