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ID 55726
フルテキストURL
著者
Okazaki, Tomoaki Graduate School of Natural Science and TechnologyOkayama University
Ichinose, Junya Graduate School of Natural Science and TechnologyOkayama University
Takebe, So Graduate School of Biology-Oriented Science and TechnologyKindai University
Ide, Toru Graduate School of Natural Science and TechnologyOkayama University
Hayakawa, Tohru Graduate School of Natural Science and TechnologyOkayama University
抄録
A Cry46Ab toxin derived from Bacillus thuringiensis strain TK-E6 shows mosquitocidal activity against Culex pipiens pallens Coquillett (Diptera: Culicidae) larvae as well as preferential cytotoxicity against human cancer cells. In B. thuringiensis cells, Cry46Ab is produced and accumulates as a protein crystal that is processed into the active 29-kDa toxin upon solubilization in the alkaline environment of the insect midgut. The Cry46Ab protoxin is 30 kDa, and is therefore thought to require an accessory protein such as P20 and/or ORF2 for efficient crystal formation. In the present study, the potency of the 4AaCter-tag was investigated for the production of alkali-soluble inclusion bodies of recombinant Cry46Ab in Escherichia coli. The 4AaCter-tag is a polypeptide derived from the C-terminal region of the B. thuringiensis Cry4Aa toxin and facilitates the formation of alkali-soluble protein inclusion bodies in E. coli. Fusion with the 4AaCter-tag enhanced both Cry46Ab production and the formation of Cry46Ab inclusion bodies. In addition, upon optimization of protein expression procedures, the Cry46Ab–4AaCter inclusion bodies showed mosquitocidal activity and stability in aqueous environments comparable to Cry46Ab without the 4AaCter-tag. Our study suggests that use of the 4AaCter-tag is a straightforward approach for preparing formulations of smaller-sized Cry toxins such as Cry46Ab in E. coli.
キーワード
Bacillus thuringiensis
Mosquitocidal Cry46Ab toxin
4AaCter-tag
Formation of protein inclusion
Escherichia coli
備考
This is an Accepted Manuscript of an article published by Springer Nature
発行日
2018-02
出版物タイトル
Applied Entomology and Zoology
53巻
1号
出版者
Springer Japan
開始ページ
67
終了ページ
73
ISSN
0003-6862
NCID
AA00543238
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
author
DOI
Web of Sience KeyUT
関連URL
isVersionOf https://doi.org/10.1007/s13355-017-0529-5