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ID 2391
Eprint ID
2391
フルテキストURL
Thumnail K002462.pdf 75.6 KB
タイトル(別表記)
一過性中大脳動脈閉塞後におけるラット脳での遊離コレステロールと中性脂質の時間的空間的変化
著者
鎌田 寛 岡山大学
抄録
In order to examine lipid metabolism in relation to neural process following brain ischemia, we investigated temporal and spatial changes of free cholesterol (FC) and neutral lipids (NLs) after 90 min of transient middle cerebral artery occlusion (MCAO). Filipin and Nile Red stainings were performed to detect mainly FC and NLs, respectively. Double stainings for Nile Red plus ED1, MAP2, or GFAP were performed in order to identify cell type of positive stainings. Filipin stanining decreased during 1–7 day and lost at 21 day after transient MCAO in the ischemic core, but did not change in the penumbra. Nile Red positive droplets reached the maximum at 7 day after transient MCAO and gradually decreased in the core, while the peak time delayed in the penumbra. MAP2 immunoreactivity lost at 7 day in the core, and increased in the penumbra during 7–56 day. Most Nile Red positive droplets were double positive for ED1 in the core, and were localized within GFAP positive cells in the penumbra. These results suggest that changes of FC and NLs are different temporally and spatially between the core and penumbra in relation to degenerative and regenerative neural processes following brain ischemia.
キーワード
Free cholesterol
Neutral lipid
Brain ischemia
Rats
備考
掲載順位 66 ; http://dx.doi.org/10.1016/S0168-0102(02)00203-1
発行日
2003-03-25
出版物タイトル
資料タイプ
学位論文
学位授与番号
甲第2462号
学位授与年月日
2003-03-25
学位・専攻分野
博士(医学)
授与大学
岡山大学
オフィシャル URL
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12507728&dopt=Abstract
言語
Japanese
OAI-PMH Set
岡山大学
論文のバージョン
none
査読
不明