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ID 2427
Eprint ID
2427
フルテキストURL
Thumnail K001694.pdf 170 KB
タイトル(別表記)
リポソームを応用した経口ワクチンの開発:経口投与可能なリポソームの脂質組成の検討ならびに経口投与によるIgA 抗体の誘導
著者
韓 梅 岡山大学
抄録
n order to evaluate the usefulness of liposomes as oral vaccines, the stability of liposomes and serum IgA antibody response to antigen associated with liposomes after oral administration were examined. Liposomes composed of dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylserine (DPPS), and cholesterol (Chol) (1:1:2, molar ratio), distearoylphosphatidylcholine (DSPC) and Chol (7:2, molar ratio), and DSPC, DPPS, and Chol (7:3:2 or 1:1:2, molar ratio) were stable in acidic solution (pH 2.0), bile, and pancreatin solution, whereas liposomes composed of DPPC and Chol (7:2, molar ratio) and DPPC, DPPS, and Chol (7:3:2, molar ratio) were unstable in pH 2.0 and/or bile solutions. After the oral immunization of antigen (ganglioside GM1)-containing liposomes composed of DPPC, DPPS, and Chol (1:1:2, molar ratio) to mice, the serum IgA antibody responses against ganglioside GM1 were found. Furthermore, when monophosphoryl lipid A was incorporated into liposomes containing ganglioside GM1, further augmentation of IgA responses to ganglioside GM1 was observed. On the other hand, the oral administration with liposomes composed of DPPC, Chol, and ganglioside GM1 (unstable liposomes), ganglioside GM1 mixed with liposomes composed of DPPC, DPPS and Chol, and ganglioside GM1 alone was unable to induce any detectable anti-ganglioside GM1 IgA antibody responses. These results suggest that liposomes which showed the stability to acidic solution, bile, and pancreatin solution would serve effectively as an oral delivery vehicle for inducing mucosal immune responses.
キーワード
liposome
mucosal immunity
oral administration
oral vaccine
stability
備考
掲載順位 69 ; http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9450240&dopt=Abstract
発行日
1998-03-25
出版物タイトル
資料タイプ
学位論文
学位授与番号
甲第1694号
学位授与年月日
1998-03-25
学位・専攻分野
博士(医学)
授与大学
岡山大学
オフィシャル URL
http://dx.doi.org/10.1292/jvms.59.1109
言語
Japanese
論文のバージョン
none
査読
不明