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ID 31301
JaLCDOI
フルテキストURL
著者
Yano, Akemi Okayama University
Yamamoto, Yuji Okayama University
Miyaishi, Satoru Okayama Univeristy
Ishizu, Hideo Okayama Uniiversity
抄録

We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp2 = 0.723 and Hp1s = 0.277. Genotyping of Hp was possible with 0.3 ng of DNA and with 0.125 microliter of blood. It was also possible with whole blood left at room temperature for a month and also with the bloodstains left at room temperature for three years. In the heated blood samples, both alleles, Hp2 and Hp1s, were detected in those heated at 100 degrees C for 2 h. In bloodstains, Hp2 and Hp1s were detected in samples heated at 100 degrees C for 2 h and 120 degrees C for 30 min. In addition, the genotype could be detected in samples other than blood such as saliva, hair roots, tissue sections and dental pulps. The present method for Hp genotyping is expected to become a useful method in forensic analysis.

キーワード
DNA polymorphism
haptoglobin
polymerase chain reaction
allele-specific amplification
personal identification
Amo Type
Article
発行日
1998-08
出版物タイトル
Acta Medica Okayama
52巻
4号
出版者
Okayama University Medical School
開始ページ
173
終了ページ
181
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT