JaLCDOI 10.18926/AMO/32744
フルテキストURL fulltext.pdf
著者 Yabe, Yoshiro| Murakami, Sakae|
抄録 <p>,The effect of infection of human embryonic skin-muscle cell cultures with adenovirus type 12 has been studied. When maintained in YLE containing 20 per cent bovine serum, human embryonic skin-muscle tissue culture cells developed little or no cytopathogenic effect for about 50 days after inoculation of adenovirus type 12, though a small amount of virus was always detected in the overlying medium. From day 50&#8764;60, CPE started appearing and spread over 90 per cent of cells accompanied with the increase of virus in the overlying medium. The addition of human serum to the maintenance medium inhibited the virus release. After removal of human serum about 16&#8764;37 days after its addition, virus-and, later, CPE also-again started appearing. The second virus release-and CPE also-was inhibited by addition of human serum to the medium. When maintained in the medium with human serum for about 200 days, the removal of human serum did not result in the appearance of virus or CPE. The virus isolated from the overlying medium of these cells during the whole process of the experiment was always highly oncogenic to newborn hamsters. Diluted adenovirus-12-immune rabbit serum also showed the effect similar to that of human serum. But, regardless of its much higher antibody titer, the effect of this diluted adenovirus-12-immune rabbit serum was weaker than that of human serum. In one of cell cultures, rapidly growing cells appeared 212 days after virus inoculation. But the available data suggest that these are the cells transformed rather spontaneously in tissue culture than by adenovirus type 12.</p>
Amo Type Article
発行日 1966-10
出版物タイトル Acta Medicinae Okayama
20巻
5号
出版者 Okayama University Medical School
開始ページ 181
終了ページ 191
NCID AA00041342
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 4227144
NAID 120002312279
JaLCDOI 10.18926/AMO/32732
フルテキストURL fulltext.pdf
著者 Yabe, Yoshiro| Ogawa, Katsuo| Iwata, Katsumi| Murakami, Sakae|
抄録 <p>Large doses of adenovirus type 12 were injected intraperitoneally into adult hamsters, and development of tumors and other pathological findings were studied in comparison with those in hamsters injected when newborn. Doses of 38~47 TCID60 per gram body weight produced tumors in 3 of 12 hamsters injected at 37~57 days of age. A dose of 170 TCID60 per gram body weight produced tumors in one of 18 hamsters injected at 61~71 days of age, but in none of 18 hamsters injected at 147~174 days of age, while the same dose per gram body weight produced tumors in 24 of 26 hamsters injected when newborn. In hamsters injected at adult ages, the number of tumors per animal decreased and the latent period for tumor development became very long as compared with those in hamsters injected when newborn. Regardless of the age at the time of injection, acute inflammatory change was observed in the peritoneum which later developed into various degrees of peritoneal adhesion. Adenovirus type 3 also induced the peritoneal adhesion. Histology of tumors was studied and discussed.</p>
Amo Type Article
発行日 1966-08
出版物タイトル Acta Medicinae Okayama
20巻
4号
出版者 Okayama University Medical School
開始ページ 147
終了ページ 154
NCID AA00041342
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 4226975
NAID 120002312084
JaLCDOI 10.18926/AMO/32688
フルテキストURL fulltext.pdf
著者 Yabe, Yoshiro|
抄録 <p>In order to study the viral etiology of human brain tumors, attempts were made to isolate cytopathogenic agents from human brain tumors by the tissue culture of tumor tissues and by the mixed culture of tumor tissues with HeLa cells. Five glioblastomas, a mixed form of glioblastoma and fibrosarcoma, two astrocytomas, two ependymomas, two meningiomas, an oligodendroglioma, a spongioblastoma polare and a choroid plexus papilloma were studied. In the tissue culture, besides the cells which appeared to be the tumor parenchymal cells, varying amounts of fibroblastic cells appeared in all the tumors tested and they increased with the prolongation of the culture period. In any of the tumors tested, no cytopathogenic agents were detected by either the culture of tumor tissues or the mixed culture of tumor tissues with HeLa cells. From the virological point of view, the significance of these negative results was discussed.</p>
Amo Type Article
発行日 1974-08
出版物タイトル Acta Medica Okayama
28巻
4号
出版者 Okayama University Medical School
開始ページ 247
終了ページ 252
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 4374049
NAID 120002311445
JaLCDOI 10.18926/AMO/31756
フルテキストURL fulltext.pdf
著者 Yabe, Yoshiro| Kuramitsu, Masae|
抄録 <p>A rapid method was devised for the detection of virus particles in wart specimens. The upper layer of warts was cut perpendicularly to the surface, and the freshly cut surface was lightly touched to an electron microscope grid. The grid was then stained with a small drop of phosphotungstate and observed electron microscopically. On the specimen grid thus prepared, papillomavirus particles were easily discriminated from tissue debris. Papillomavirus particles were detected in 71% of verrucae plantares, 78% of verrucae palmares, 50% of verrucae vulgares and 75% of condylomata acuminata by the present method.</p>
キーワード papillomavirus warts electron microscopy
Amo Type Brief Note
発行日 1987-10
出版物タイトル Acta Medica Okayama
41巻
5号
出版者 Okayama University Medical School
開始ページ 233
終了ページ 235
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2825468
Web of Science KeyUT A1987K590100008
JaLCDOI 10.18926/AMO/31148
フルテキストURL fulltext.pdf
著者 Yabe, Yoshiro| Koyama, Hiroko|
抄録 <p>A subcutaneous tumor of a patient with epidermodysplasia verruciformis was studied by the light microscopy, the electronmicroscopy and the immunofluorescent test. The tumor cells were histologically pleomorphic and electronmicroscopically contained varying amounts of cytoplasmic filaments without Z-band formation. The antimyosin serum stained the tumor cells, showing their myogenic origin. No virus or virus-like particles were observed in the tumor. Tumor antigens stainable by the patient's serum were not detected. Hamsters inoculated with the tumor extract at birth developed no noticeable diseases.</p>
Amo Type Article
発行日 1971-12
出版物タイトル Acta Medicinae Okayama
25巻
6号
出版者 Okayama University Medical School
開始ページ 643
終了ページ 648
NCID AA00041342
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 4264056
NAID 120002313000
JaLCDOI 10.18926/AMO/31040
フルテキストURL fulltext.pdf
著者 Yabe, Yoshiro| Matsumoto, Kyoko| Ogura, Hajime|
抄録 <p>To establish an experimental persistent infection of the brain with human adenoviruses, adenovirus type 6 (ad 6) was inoculated intracerebrally into young adult hamsters. Hamsters appeared languid for a few days after inoculation, but recovered rapidly. By cocultivation of tissue fragments with HeLa cells, ad 6 was always recovered from the brains of hamsters throughout their lives, as long as 29 months, indicating the establishment of a lifelong persistent infection. Except for the first few days after inoculation, however, attempts to recover virus by inoculation of tissue extracts onto HeLa cells or by cultivation of tissue fragments alone were unsuccessful.</p>
キーワード adenovirus type 6 persistent infection hamster brain
Amo Type Article
発行日 1988-02
出版物タイトル Acta Medica Okayama
42巻
1号
出版者 Okayama University Medical School
開始ページ 45
終了ページ 47
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 3364214
Web of Science KeyUT A1988M237800008
JaLCDOI 10.18926/AMO/30999
フルテキストURL fulltext.pdf
著者 Yabe, Yoshiro| Sakai, Akiko| Tanimura, Yoshie| Kuramitsu, Masae| Hitsumoto, Takako| Ishii, Kanji| Ueki, Hiroaki|
抄録 <p>Two distinct human papillomavirus (HPV) DNAs (MY-1 and MY-2) were molecularly cloned from the benign skin lesions of a patient with epidermodysplasia verruciformis. The restriction map of MY-1 was the same as that of HPV 3a. The map of MY-2 appeared to be different from those of any HPVs reported in the literature. MY-2 did not cross-hybridize with MY-1 or the DNAs of HPV types 1, 2 and 4 under stringent conditions.</p>
キーワード papillomavirus viral DNA molecular cloning restriction map epidermodysplasia verruciformis
Amo Type Article
発行日 1988-08
出版物タイトル Acta Medica Okayama
42巻
4号
出版者 Okayama University Medical School
開始ページ 243
終了ページ 245
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2845712
Web of Science KeyUT A1988P884600008