JaLCDOI 10.18926/AMO/30881
フルテキストURL fulltext.pdf
著者 Masuoka, Noriyoshi| Ubuka, Toshihiko| Akagi, Reiko| Yao, Kenzaburoh| Ishino, Kazushi|
抄録 <p>A new volatile derivative of taurine, N-isobutoxycarbonyltaurine methyl ester (methyl 2-(N-isobutoxycarbonylamino)ethanesulfonate), was prepared by a three-step procedure for the gas chromatographic determination of taurine in urine. First, taurine was converted to its silver salt by reaction with silver oxide; next the silver salt was reacted with isobutyl chloroformate to form the N-isobutoxycarbonyl derivative, and finally the derivative was reacted with methyl iodide to form N-isobutoxycarbonyltaurine methyl ester. The volatile derivative was analyzed by gas chromatography using a column of 3% OV-101 on Chromosorb W. When methyl 3-(N-isobutoxycarbonylamino) propanesulfonate was used as an internal standard, the calibration curve was linear between 0.5 and 5.0 mumol of taurine/ml and showed a good reproducibility. This method was applied to the determination of taurine in human urine. Recovery was 98.6 +/- 5.2%, when 1.25 to 5.0 mumol/ml of taurine was added to human urine.</p>
キーワード taurine gas chromatography taurine determination methyI 2-(N-isobutoxycarbonylamino) ethanesulfoate taurine excretion
Amo Type Article
発行日 1989-10
出版物タイトル Acta Medica Okayama
43巻
5号
出版者 Okayama University Medical School
開始ページ 253
終了ページ 259
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2610003
Web of Science KeyUT A1989CA06200001
JaLCDOI 10.18926/AMO/30459
フルテキストURL fulltext.pdf
著者 Yuasa, Shigeki| Akagi, Reiko| Ubuka, Toshihiko| Masuoka, Noriyoshi| Yao, Kenzaburoh|
抄録 <p>The excretion of 3-mercaptolactate-cysteine mixed disulfide [S-(2-hydroxy-2-carboxyethylthio)-L-cysteine, HCETC], sulfate and taurine in the urine of normal adults was investigated before and after oral administration of L-cysteine and related sulfur-containing amino acids. Before the loading of amino acids, the excretion (mean +/- SD) per kg of body weight per day of HCETC, free sulfate and taurine was 0.096 +/- 0.042, 305.7 +/- 66.1 and 31.9 +/- 8.7 mumols, respectively. After the loading of L-cysteine (800 mumols/kg of body weight), the average excretion in the 24-h urine of HCETC increased 2-fold and that of taurine increased 1.6-fold. The average excretion of free sulfate after the L-cysteine loading was 989.4 +/- 145.1 and 388.8 +/- 51.6 mumols/kg per day in the first and second 24-h urine, respectively, indicating that the sulfur corresponding to 85% of the L-cysteine loaded was excreted as free sulfate in 24 h. Administration of L-cystine (400 mumols/kg) resulted in similar results. The increase in HCETC after L-cysteine or L-cystine administration indicates that L-cysteine is metabolized in part through the transamination pathway (3-mercaptopyruvate pathway) and that an equilibrium exists between the intake and excretion of sulfur in humans.</p>
キーワード 3-mercaptolactate-cysteine mixed disulfide cysteine matabolism 3-mercaptopyruvate pathway sulfur amino acid sulfate excretion
Amo Type Article
発行日 1990-06
出版物タイトル Acta Medica Okayama
44巻
3号
出版者 Okayama University Medical School
開始ページ 117
終了ページ 122
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2382576
Web of Science KeyUT A1990DM18300001
JaLCDOI 10.18926/AMO/30442
フルテキストURL fulltext.pdf
著者 Ubuka, Toshihiko| Yuasa, Shigeki| Ohta, Jun| Masuoka, Noriyoshi| Yao, Kenzaburoh| Kinuta, Masahiro|
抄録 <p>Formation of sulfate in rat liver mitochondria was studied. About 0.1 mumol of sulfate was formed in mitochondria from 1 g of liver in 60 min when 10 mM L-cysteine was used as the substrate. Addition of either 10 mM 2-oxoglutarate or 10 mM glutathione to this system increased sulfate formation 3 to 4 times. The addition of both 2-oxoglutarate and glutathione resulted in a 20-fold increase in sulfate formation. Sulfate formation in the presence of 5 mM L-cysteine was 58% of that with 10 mM L-cysteine. L-Cysteine-glutathione mixed disulfide was not a good substrate, indicating that this mixed disulfide was not an intermediate of sulfate formation in the present system. Incubation of 3-mercaptopyruvate with rat liver mitochondria also resulted in sulfate formation, and the addition of glutathione accelerated it. Formation of sulfite and thiosulfate was also detected. These results indicate that sulfate is produced in mitochondria, at least in part, from L-cysteine through the transamination pathway (3-mercaptopyruvate pathway).</p>
キーワード sulfate formatioon cysteine metabolism glutathione 3-mercaptopyruvate mitochondria
Amo Type Article
発行日 1990-04
出版物タイトル Acta Medica Okayama
44巻
2号
出版者 Okayama University Medical School
開始ページ 55
終了ページ 64
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2363365
Web of Science KeyUT A1990DE10000001