JaLCDOI 10.18926/AMO/30439
フルテキストURL fulltext.pdf
著者 Yao, Kenzabroh| Ubuka, Toshihiko| Masuoka, Noriyoshi| Kinuta, Masahiro| Ohta, Jun| Ishino, Kazushi|
抄録 <p>A new method for staining sialoglycoproteins in polyacrylamide gel after disc electrophoresis is described. The method utilizes the reaction of sialic acids with an acidic ninhydrin reagent which yields a stable color with an absorption maximum at 470 nm. After electrophoresis, the polyacrylamide gel is placed in a test tube and heated with 5 ml of the acidic ninhydrin reagent for 10 min in a boiling water bath. Sialoglycoproteins are detected as brown bands. No additional procedure such as destaining is necessary. When 20 micrograms fetuin, a sialoglycoprotein, per gel is applied, the band remains visible for at least 2 h. Stained gel can be scanned with a gel scanner at 470 nm. When the stained gel was dried on a sheet of polypropylene filter, the color was stable for at least one month. The present method is superior to the method using Stains-all (3,3'-diethyl-9-methyl-4,5,4',5'-dibenzothiacarbocyanine) in specificity and simplicity for the detection of sialoglycoproteins.</p>
キーワード sialoglycoprotein polyacrylamide gel electrophoresis staining acidic ninhydrin reaction
Amo Type Article
発行日 1990-04
出版物タイトル Acta Medica Okayama
44巻
2号
出版者 Okayama University Medical School
開始ページ 65
終了ページ 70
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1694626
Web of Science KeyUT A1990DE10000002
JaLCDOI 10.18926/AMO/31741
フルテキストURL fulltext.pdf
著者 Yao, Kenzabroh| Ubuka, Toshihiko|
抄録 <p>A new acidic ninhydrin method for determining free sialic acids is described. The method is based on the reaction of sialic acids with Gaitonde's acid ninhydrin reagent 2 which yields a stable color with an absorption maximum at 470 nm. The standard curve is linear in the range of 5 to 500 nmol of N-acetylneuraminic acid per 0.9 ml of reaction mixture. The reaction was specific only for sialic acids among the various sugars and sugar derivatives examined. Some interference of this method by cysteine, cystine and tryptophan was noted, although their absorption maxima differed from that of sialic acids. The interference by these amino acids was eliminated with the use of a small column of cation-exchange resin. The acidic ninhydrin method provides a simple and rapid method for the determination of free sialic acids in biological materials.</p>
キーワード sialic acid determination acidic ninhydrin reaction acidic ninhydrin method
Amo Type Article
発行日 1987-12
出版物タイトル Acta Medica Okayama
41巻
6号
出版者 Okayama University Medical School
開始ページ 237
終了ページ 241
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 3439478
Web of Sience KeyUT A1987L530300001