このエントリーをはてなブックマークに追加
ID 17114
Eprint ID
17114
フルテキストURL
Thumnail 92_923.pdf 4.63 MB
タイトル(別表記)
Molecular forms of SV40 chromatin and their functional changes
著者
渡邊 晰子 岡山大学医学部癌源研究施設生化学部
抄録
Simian virus 40 nucleoprotein complexes (SV40 chromatin) have a nucleosome structure similar to that of cellular chromatin and serve as a useful model system for studying structure and function of cellular chromatin. It is the purpose of this study to clarify the molecular forms of SV40 chromatin and their functional changes. African green monkey kidney cells (CV-1) were infected with SV40 and bulk or pulse-labeled with [(3)H] thymidine. SV40 chromatin was isolated under various conditions from these cell nuclei and examined by electron microscopy. When SV40 chromatin was extracted with a hypertonic salt-Triton X-100 solution from the nuclei isolated by treatment with a hypotonic ethylenediaminetetraacetic acid (EDTA) solution, the majority of SV40 chromatin molecules had a relaxed form showing a typical bead-like structure of nucleosomes. Most of these molecules were found to be derived from disrupted virions. When the extraction was performed under mild conditions in a near isotonic solution by no pretreatment with EDTA, SV40 chromatin and virions were separated in different fractions by sucrose density gradient centrifugation. The majority of these SV40 chromatin molecules had a condensed form. These molecules can be relaxed through various intermediate forms by treatment with a hypotonic or 0.6M NaCl solution. Replicating SV40 chromatin was sedimented faster than mature SV40 chromatin and showed the relaxed form. SV40 chromatin isolated from purified virion by alkali treatment showed the relaxed form. The relaxed molecules were condensed and their template activity for transcription was strongly inhibited by binding with histone Hl.
キーワード
SV40 クロマチン
ヌクレオソーム構造
分子形態
複製形
転写調節
発行日
1980-08-31
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
92巻
7-8号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
923
終了ページ
941
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/92/7-8/92_7-8_923/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma