JaLCDOI 10.18926/AMO/31323
フルテキストURL fulltext.pdf
著者 Wakimoto, Masahiro| Masuoka, Noriyoshi| Nakano, Taku| Ubuka, Toshihiko|
抄録 <p>A new method for the determination of glutathione peroxidase activity in erythrocytes was developed. The present method was applied to the measurement of hydrogen peroxide removal rates by glutathione peroxidase in erythrocytes at 70 microM hydrogen peroxide under simulated in vivo conditions. The removal rates by glutathione peroxidase in mouse erythrocytes were twenty-times faster than those in human ones and were 5.2 mumol/sec/g of Hb. The removal rates in acatalasemic mouse erythrocytes indicate that glutathione peroxidase is the main means of hydrogen peroxide removal in acatalasemic mouse erythrocytes. Based on these results, we concluded that glutathione peroxidase in mouse erythrocytes had sufficient ability to remove hydrogen peroxide at even relatively high concentrations. This may be one of the reasons why acatalasemic mice suffer no health problems while Japanese acatalasemic patients suffer from Takahara disease when infected with hydrogen peroxide-generating bacteria.</p>
キーワード glutathione peroxidase erythrocyte hydrogen peroxide acatalasemic mouse Takahara disease
Amo Type Article
発行日 1998-10
出版物タイトル Acta Medica Okayama
出版者 Okayama University Medical School
開始ページ 233
終了ページ 237
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9810432
Web of Science KeyUT 000076694300001