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ID 31323
JaLCDOI
フルテキストURL
著者
Wakimoto, Masahiro Okayama University
Masuoka, Noriyoshi Okayama University
Nakano, Taku Toyama Medical and Pharmaceutical University
Ubuka, Toshihiko Okayama University
抄録

A new method for the determination of glutathione peroxidase activity in erythrocytes was developed. The present method was applied to the measurement of hydrogen peroxide removal rates by glutathione peroxidase in erythrocytes at 70 microM hydrogen peroxide under simulated in vivo conditions. The removal rates by glutathione peroxidase in mouse erythrocytes were twenty-times faster than those in human ones and were 5.2 mumol/sec/g of Hb. The removal rates in acatalasemic mouse erythrocytes indicate that glutathione peroxidase is the main means of hydrogen peroxide removal in acatalasemic mouse erythrocytes. Based on these results, we concluded that glutathione peroxidase in mouse erythrocytes had sufficient ability to remove hydrogen peroxide at even relatively high concentrations. This may be one of the reasons why acatalasemic mice suffer no health problems while Japanese acatalasemic patients suffer from Takahara disease when infected with hydrogen peroxide-generating bacteria.

キーワード
glutathione peroxidase
erythrocyte
hydrogen peroxide
acatalasemic mouse
Takahara disease
Amo Type
Article
発行日
1998-10
出版物タイトル
Acta Medica Okayama
52巻
5号
出版者
Okayama University Medical School
開始ページ
233
終了ページ
237
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Science KeyUT