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ID 31741
JaLCDOI
フルテキストURL
著者
Yao, Kenzabroh Okayama University
Ubuka, Toshihiko Okayama University
抄録

A new acidic ninhydrin method for determining free sialic acids is described. The method is based on the reaction of sialic acids with Gaitonde's acid ninhydrin reagent 2 which yields a stable color with an absorption maximum at 470 nm. The standard curve is linear in the range of 5 to 500 nmol of N-acetylneuraminic acid per 0.9 ml of reaction mixture. The reaction was specific only for sialic acids among the various sugars and sugar derivatives examined. Some interference of this method by cysteine, cystine and tryptophan was noted, although their absorption maxima differed from that of sialic acids. The interference by these amino acids was eliminated with the use of a small column of cation-exchange resin. The acidic ninhydrin method provides a simple and rapid method for the determination of free sialic acids in biological materials.

キーワード
sialic acid determination
acidic ninhydrin reaction
acidic ninhydrin method
Amo Type
Article
発行日
1987-12
出版物タイトル
Acta Medica Okayama
41巻
6号
出版者
Okayama University Medical School
開始ページ
237
終了ページ
241
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT