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ID 52009
JaLCDOI
フルテキストURL
Thumnail 67_6_359.pdf 2.12 MB
著者
Katashima, Kazunori Department of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kuroda, Masahiro Radiological Technology, Graduate School of Health Sciences, Okayama University
Ashida, Masakazu Department of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Sasaki, Takanori Department of Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Taguchi, Takehito Radiological Technology, Graduate School of Health Sciences, Okayama University
Matsuzaki, Hidenobu Department of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Murakami, Jun Department of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Yanagi, Yoshinobu Department of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Hisatomi, Miki Department of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Hara, Marina Department of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kato, Hirokazu Radiological Technology, Graduate School of Health Sciences, Okayama University
Ohmura, Yuichi Radiological Technology, Graduate School of Health Sciences, Okayama University
Kobayashi, Tomoki Radiological Technology, Graduate School of Health Sciences, Okayama University
Kanazawa, Susumu Department of Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Harada, Sosuke Department of Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Takemoto, Mitsuhiro Department of Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Ohno, Seiichiro Central Division of Radiology, Okayama University Hospital
Mimura, Seiichi Central Division of Radiology, Okayama University Hospital
Asaumi, Junichi Department of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
抄録
It is well known that many tumor tissues show lower apparent diffusion coefficient (ADC) values, and that several factors are involved in the reduction of ADC values. The aim of this study was to clarify how much each factor contributes to decreases in ADC values. We investigate the roles of cell density, extracellular space, intracellular factors, apoptosis and necrosis in ADC values using bio-phantoms. The ADC values of bio-phantoms, in which Jurkat cells were encapsulated by gellan gum, were measured by a 1.5-Tesla magnetic resonance imaging device with constant diffusion time of 30sec. Heating at 42℃ was used to induce apoptosis while heating at 48℃ was used to induce necrosis. Cell death after heating was evaluated by flow cytometric analysis and electron microscopy. The ADC values of bio-phantoms including non-heated cells decreased linearly with increases in cell density, and showed a steep decline when the distance between cells became less than 3μm. The analysis of ADC values of cells after destruction of cellular structures by sonication suggested that approximately two-thirds of the ADC values of cells originate from their cellular structures. The ADC values of bio-phantoms including necrotic cells increased while those including apoptotic cells decreased. This study quantitatively clarified the role of the cellular factors and the extracellular space in determining the ADC values produced by tumor cells. The intermediate diffusion time of 30msec might be optimal to distinguish between apoptosis and necrosis.
キーワード
ADC
apoptosis
necrosis
hyperthermia
cell density
Amo Type
Original Article
発行日
2013-12
出版物タイトル
Acta Medica Okayama
67巻
6号
出版者
Okayama University Medical School
開始ページ
359
終了ページ
367
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
著作権者
CopyrightⒸ 2013 by Okayama University Medical School
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT