JaLCDOI 10.18926/AMO/30788
フルテキストURL fulltext.pdf
著者 Matsuo, Toshihiko| Takabatake, Mayumi| Matsuo, Nobuhiko|
抄録 <p>Retinal cells from chick embryos aged 7.5 days of gestation were cultured for two months in a non-adherent suspension culture dish to study the effects of growth factors and co-culture with retinal pigment epithelial cells on their differentiation. Dissociated retinal cells became cellular aggregates (multicellular spheroids) within a day, and rosettes were formed in the spheroids after 2 days. Ultrastructurally, neurons of the rosettes developed connecting cilia, ellipsoids (accumulation of mitochondria), and external limiting membrane, indicative of their differentiation into photoreceptor cells. Epidermal growth factor enhanced the expression of rhodopsin by rosette-forming neurons, while basic fibroblast growth factor induced the growth of Mueller cells at 4 weeks, and their transdifferentiation into lens-epithelial-like cells at 8 weeks. Co-culture of retinal cells with retinal pigment epithelial cells enhanced the formation of rosettes in spheroids. Multicellular spheroids formed in a dish for suspension culture would provide a convenient in vitro system to examine differentiation and transdifferentiation of the retina.</p>
キーワード multicellular spheroids differentiation transdifferentiation retina retinal pigment epithelium growth factors
Amo Type Article
発行日 1997-10
出版物タイトル Acta Medica Okayama
出版者 Okayama University Medical School
開始ページ 251
終了ページ 260
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9359922
Web of Science KeyUT A1997YD65300003