Matsuura, Koji Research Core for Interdisciplinary Sciences, Okayama University
Hayashi, Nobuyoshi Okayama Couples Clinic
Kuroda, Yuka Research Core for Interdisciplinary Sciences, Okayama University
Takiue, Chisato Okayama Couples Clinic
Hirata, Rei Okayama Couples Clinic
Takenami, Mami Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Aoi, Yoko Okayama Couples Clinic
Yoshioka, Nanako Okayama Couples Clinic
Habara, Toshihiro Okayama Couples Clinic
Mukaida, Tetsunori Hiroshima HART Clinic
Naruse, Keiji Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Mammalian embryos experience not only hormonal but also mechanical stimuli, such as shear stress, compression and friction force in the Fallopian tube before nidation. In order to apply mechanical stimuli to embryos in a conventional IVF culture system, the tilting embryo culture system (TECS) was developed. The observed embryo images from the TECS suggest that the velocities and shear stresses of TECS embryos are similar to those experienced in the oviduct. Use of TECS enhanced the development rate to the blastocyst stage and significantly increased the cell number of mouse blastocysts (P < 0.05). Although not statistically significant, human thawed embryos showed slight improvement in development to the blastocyst stage following culture in TECS compared with static controls. Rates of blastocyst formation following culture in TECS were significantly improved in low-quality embryos and those embryos cultured under suboptimal conditions (P < 0.05). The TECS is proposed as a promising approach to improve embryo development and blastocyst formation by exposing embryos to mechanical stimuli similar to those in the Fallopian tube.
tilting embryo culture system
Reproductive BioMedicine Online
Copyright © 2009 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
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