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ID 31649
JaLCDOI
フルテキストURL
著者
Qiu, Yue Kochi Medical School
Takeuchi, Tamotsu Kochi Medical School
Chen, Bing-Kun Kochi Medical School
Ohtsuki, Yuji Kochi Medical School
抄録

We applied an in situ polymerase chain reaction (PCR) hybridization method in order to detect human T-lymphotropic virus type I-infected cells in routinely-processed paraffin sections of the lung from 13 autopsied patients with adult T-cell leukemia (ATL). Previously reported protocol resulted in somewhat non-specific staining in our sections. Therefore, we used a hot start PCR method using specialized commercially-available polymerase in order to increase the specificity. Of 6 patients with ATL cell invasion into the lungs, 4 exhibited strong positive staining of almost all invading ATL cells. In contrast, 7 patients without ATL cell invasion into the lungs did not demonstrate any significant reactivity. Since the method described here is a relatively simple hot start method and does not yield false-positives, it may allow us to determine whether human T-lymphotropic virus type I (HTLV-I) associated disorders are related to lymphocytes integrating the HTLV-I genome.

キーワード
polymerase chain reaction
in situ hybridization
human T-lymphotopic virus type I
paraffin section
lung
Amo Type
Article
発行日
1999-02
出版物タイトル
Acta Medica Okayama
53巻
1号
出版者
Okayama University Medical School
開始ページ
1
終了ページ
4
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT