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ID 56674
フルテキストURL
著者
Senoh, Mitsutoshi Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
Ghosh-Banerjee, Jayeeta National Institute of Cholera and Enteric Diseases,
Mizuno, Tamaki Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
Shinoda, Sumio Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
Miyoshi, Shin-ichi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Hamabata, Takashi Research Institute, National Center for Global Health and Medicine
Nair, G. Balakrish Translational Health Science and Technology Institute
Takeda, Yoshifumi Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
抄録
Previously, we reported that viable but nonculturable (VBNC) Vibrio cholerae was converted into a culturable state by coculture with several eukaryotic cell lines including HT-29 cells. In this study, we found that a factor converting VBNC V. cholerae into a culturable state (FCVC) existed in cell extracts of eukaryotic cells. FCVC was nondialyzable, proteinase K-sensitive, and stable to heating at <60°C for 5 min. We prepared thiosulfate citrate bile salts sucrose (TCBS) plates with FCVC (F-TCBS plates). After confirming that VBNC V. cholerae O1 and O139 formed typical yellow colonies on F-TCBS plates, we tried to isolate cholera toxin gene-positive VBNC V. cholerae from environmental water samples collected in urban slum areas of Kolkata, India and succeeded in isolating V. cholerae O1 El Tor variant strains harboring a gene for the cholera toxin. The possible importance of VBNC V. cholerae O1 as a source of cholera outbreaks is discussed.
キーワード
Factor converting VBNC into culturable
VBNC Vibrio cholerae
発行日
2014-02-18
出版物タイトル
Microbiologyopen
3巻
2号
出版者
Wiley-Blackwell
開始ページ
239
終了ページ
246
ISSN
20458827
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
publisher
PubMed ID
DOI
Web of Sience KeyUT
関連URL
isVersionOf https://doi.org/10.1002/mbo3.164
プロジェクト
インド感染症共同研究