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ID 47723
フルテキストURL
著者
Maekawa, Tetsuya Department of Biology, Graduate School of Natural Science and Technology, Okayama University
Takeuchi, Sakae Department of Biology, Graduate School of Natural Science and Technology, Okayama University
Kanayama, Munetoshi Department of Biology, Graduate School of Natural Science and Technology, Okayama University
Takahashi, Sumio Department of Biology, Graduate School of Natural Science and Technology, Okayama University
抄録
Insulin-like growth factor 1 (IGF1) Is Involved in the proliferation of mouse and rat endometrial cells in a paracrine or autocrine manner. Insulin-like growth factor binding protein-3 (IGFBP3) modulates actions of IGFs directly or indirectly. The present study aimed to determine whether IGFBP3 is Involved In the regulation of proliferation of mouse endometrial cells. Mouse endometrial epithelial cells and stromal cells were isolated, and cultured In a serum free medium. IGF1 stimulated DNA synthesis by endometrial epithelial and stromal cells, and IGFBP3 Inhibited IGF1-induced DNA synthesis. Estradiol-17 beta (E2) decreased the Igfbp3 mRNA level in endometrial stromal cells, whereas It Increased the Igf1 mRNA level. Transforming growth factor alpha (TGF alpha) significantly decreased IGFBP3 expression at both the mRNA and secreted protein levels in endometrial stromal cells. Progesterone (134) did not affect the E2-induced down-regulation of Igfbp3 mRNA expression in endometrial stromal cells, although P4 alone increased Igfbp3 mRNA levels. The present findings suggest that in mouse endometrial stromal cells E2 enhances IGF1 action through enhancement of IGF1 synthesis and reduction of IGFBP3 synthesis, and that TGF alpha affects IGF1 actions through modulation of IGFBP3 levels.
キーワード
IGFBP3
IGF1
estrogen
mouse
uterus
発行日
2009-02
出版物タイトル
Zoological Science
26巻
2号
出版者
Zoological Society of Japan
出版者(別表記)
日本動物学会
開始ページ
131
終了ページ
138
ISSN
0289-0003
NCID
AA10545874
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
© 2009 Zoological Society of Japan
論文のバージョン
publisher
査読
有り
DOI
PubMed ID
Web of Sience KeyUT