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ID 47726
フルテキストURL
著者
Kusumoto, Kenji Department of Biology, Faculty of Science, Okayama University
Murakami, Yousuke Department of Biology, Faculty of Science, Okayama University
Otsuki, Mariko Department of Biology, Faculty of Science, Okayama University
Kanayama, Munetoshi Tsushima Facility, Advanced Science Research Center, Okayama University
Takeuchi, Sakae Department of Biology, Faculty of Science, Okayama University
Takahashi, Sumio Department of Biology, Faculty of Science, Okayama University
抄録
Interleukin-18 (IL-18) belongs to the interleukin-1 family and was identified as an interferon gamma inducing factor. We investigated IL-18 mRNA-expressing cells in the mouse uterus. By RNase protection assay, IL-18 mRNA and a subunit of IL-18 receptor mRNA were detected in the uterus. In the uterus, IL-18 mRNA levels increased during sexual maturation. In situ hybridization analysis demonstrated IL-18 mRNA-expressing cells in the mouse uterus of different ages. At 21 days of age, IL-18 mRNA-expressing cells were detected in the luminal epithelial cells and stromal cells although the IL-18 mRNA signal was weak. At 42 days of age, IL-18 mRNA signal was mainly detected in the stromal cells located near the myometrium, and in some of the luminal and glandular epithelial cells. In the uterus of 63-day-old adult mice, a strong hybridization signal for IL-18 mRNA was detected at estrus, but was weak at diestrus. IL-18 mRNA was mainly detected in the glandular epithelial cells and stromal cells. The effect of estradiol-17 beta (E-2) on IL-18 mRNA-expressing cells in the uterus was examined in ovariectomized mice. In oil-treated mice IL-18 mRNA signal was localized in luminal epithelial cells and stromal cells, while in E-2-treated mice IL-18 mRNA signal was localized in stromal cells alone. These results suggest that the mouse uterus has an IL-18 system, and IL-18 exerts a physiological role within the uterus in a paracrine manner, and that IL-18 gene expression is regulated by estrogen.
キーワード
interleukin-18 (IL-18)
uterus
estrogen
mouse
発行日
2005-09
出版物タイトル
Zoological Science
22巻
9号
出版者
Zoological Society of Japan
出版者(別表記)
日本動物学会
開始ページ
1003
終了ページ
1010
ISSN
0289-0003
NCID
AA10545874
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
© 2005 Zoological Society of Japan
論文のバージョン
publisher
査読
有り
DOI
PubMed ID
Web of Sience KeyUT