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ID 49681
フルテキストURL
著者
Tanaka, Shigetomi Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
Shiraha, Hidenori Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
Nakanishi, Yutaka Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
Nishina, Shin-Ichi Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
Matsubara, Minoru Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
Horiguchi, Shigeru Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
Takaoka, Nobuyuki Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
Iwamuro, Masaya Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
Kataoka, Junro Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
Kuwaki, Kenji Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
Hagihara, Hiroaki Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
Toshimori, Junichi Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
Ohnishi, Hideki Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
Takaki, Akinobu Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
Nakamura, Shinichiro Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
Nouso, Kazuhiro Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
Yagi, Takahito Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol Surg Transplant & Surg Oncol
Yamamoto, Kazuhide Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
抄録
Loss or decreased expression of runt-related transcription factor 3 (RUNX3), a tumor suppressor gene involved in gastric and other cancers, has been frequently observed in hepatocellular carcinoma (HCC). The objective of this study was to identify the regulatory mechanism of the epithelialmesenchymal transition (EMT) by RUNX3 in HCC. Human HCC cell lines, Hep3B, Huh7, HLF and SK-Hep1, were divided into low- and high-EMT lines, based on their expression of TWIST1 and SNAI2, and were used in this in vitro study. Ectopic RUNX3 expression had an anti-EMT effect in low-EMT HCC cell lines characterized by increased E-cadherin expression and decreased N-cadherin and vimentin expression. RUNX3 expression has previously been reported to reduce jagged-1 (JAG1) expression; therefore, JAG1 ligand peptide was used to reinduce EMT in RUNX3-expressing low-EMT HCC cells. Immunohistochemical analyses were performed for RUNX3, E-cadherin, N-cadherin and TWIST1 in 33 human HCC tissues, also divided into low- and high-EMT HCC, based on TWIST1 expression. E-cadherin expression was correlated positively and N-cadherin expression was correlated negatively with RUNX3 expression in low-EMT HCC tissues. Correlations between EMT markers and RUNX3 mRNA expression were analyzed using Oncomine datasets. Similarly, mRNA expression of E-cadherin was also significantly correlated with that of RUNX3 in low-EMT HCC, while mRNA expression of JAG1 was negatively correlated with that of RUNX3. These results suggest a novel mechanism by which loss or decreased expression of RUNX3 induces EMT via induction of JAG1 expression in low-EMT HCC.
キーワード
cell migration
tumor invasion
jagged-1
E-cadherin
N-cadherin
発行日
2012-12-01
出版物タイトル
International Journal of Cancer
131巻
11号
開始ページ
2537
終了ページ
2546
ISSN
0020-7136
NCID
AA00680002
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1002/ijc.27575
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/49134
言語
English
著作権者
© 2012 UICC
論文のバージョン
author
査読
有り
DOI
Web of Sience KeyUT