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ID 56702
フルテキストURL
著者
Sinha, A. National Institute of Cholera and Enteric Diseases (NICED)
SenGupta, S. National Institute of Cholera and Enteric Diseases (NICED)
Guin, S. National Institute of Cholera and Enteric Diseases (NICED)
Dutta, S. National Institute of Cholera and Enteric Diseases (NICED)
Ghosh, S. National Institute of Cholera and Enteric Diseases (NICED)
Mukherjee, P. National Institute of Cholera and Enteric Diseases (NICED)
Mukhopadhyay, A. K. National Institute of Cholera and Enteric Diseases (NICED)
Ramamurthy, T. National Institute of Cholera and Enteric Diseases (NICED)
Takeda, Y. Collaborative Research Centre of Okayama University for Infectious Diseases in India, NICED
Kurakawa, T. Yakult Central Institute for Microbiological Research
Nomoto, K. Yakult Central Institute for Microbiological Research
Nair, G. B. National Institute of Cholera and Enteric Diseases (NICED)
Nandy, R. K. National Institute of Cholera and Enteric Diseases (NICED)
抄録
Culture-independent identification of diarrhoeal aetiological agents was performed using DNA harvested from diarrhoeal stool specimens with SYBR-Green-based real-time PCR targeting Vibrio cholerae, Vibrio parahaemolyticus, Campylobacter spp., Shigella spp. and three different pathotypes of diarrhoeagenic Escherichia coli. Conventional culture-dependent methods detected bacterial enteropathogens in 68 of 122 diarrhoeal stool specimens. Of 68 specimens, 59 (86.8%) had a single pathogen and the remaining nine (13.2%) had polymicrobial infections with multiple pathogens. Re-analysis of the 68 specimens by culture-independent real-time PCR methods showed that 25 (36.8%) specimens contained single pathogen and 43 (63.2%) specimens contained mixed infections with multiple pathogens. The prevalence of such high levels of polymicrobial infections would not have been detected without using real-time PCR. Culture-dependent analysis assigned 54 of the 122 selected archived specimens as 'no known aetiology'. However, re-analysis of these samples by real-time PCR showed the presence of single or multiple pathogens among 34 (63%) of these specimens. Estimation of relative pathogen load by real-time PCR in the stool specimens indicated that the inability of conventional culture-dependent methods to detect the pathogens was related to lower colony-forming units of the pathogen, as reflected by lower C(t) values. Detection of high levels of polymicrobial infection by real-time PCR indicates that in the settings like Kolkata and its surroundings, where cholera and other enteric diseases are endemic, the concept of one pathogen one disease might need to be re-evaluated.
キーワード
Real-time PCR
Diarrhoea
Polymicrobial infection
備考
This is an Accepted Manuscript of an article published by Elsevier B.V
発行日
2013-02
出版物タイトル
Clinical Microbiology and Infection
19巻
2号
出版者
Elsevier B.V
開始ページ
173
終了ページ
180
ISSN
1198743X
NCID
AA11098239
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
author
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1111/j.1469-0691.2011.03746.x
プロジェクト
インド感染症共同研究