| JaLCDOI | 10.18926/AMO/31649 |
|---|---|
| フルテキストURL | fulltext.pdf |
| 著者 | Qiu, Yue| Takeuchi, Tamotsu| Chen, Bing-Kun| Ohtsuki, Yuji| |
| 抄録 | <p>We applied an in situ polymerase chain reaction (PCR) hybridization method in order to detect human T-lymphotropic virus type I-infected cells in routinely-processed paraffin sections of the lung from 13 autopsied patients with adult T-cell leukemia (ATL). Previously reported protocol resulted in somewhat non-specific staining in our sections. Therefore, we used a hot start PCR method using specialized commercially-available polymerase in order to increase the specificity. Of 6 patients with ATL cell invasion into the lungs, 4 exhibited strong positive staining of almost all invading ATL cells. In contrast, 7 patients without ATL cell invasion into the lungs did not demonstrate any significant reactivity. Since the method described here is a relatively simple hot start method and does not yield false-positives, it may allow us to determine whether human T-lymphotropic virus type I (HTLV-I) associated disorders are related to lymphocytes integrating the HTLV-I genome.</p> |
| キーワード | polymerase chain reaction in situ hybridization human T-lymphotopic virus type I paraffin section lung |
| Amo Type | Article |
| 発行日 | 1999-02 |
| 出版物タイトル | Acta Medica Okayama |
| 巻 | 53巻 |
| 号 | 1号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 1 |
| 終了ページ | 4 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | English |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 10096731 |
| Web of Sience KeyUT | 000078897700001 |