JaLCDOI |
10.18926/AMO/31649
|
フルテキストURL |
fulltext.pdf
|
著者 |
Qiu, Yue|
Takeuchi, Tamotsu|
Chen, Bing-Kun|
Ohtsuki, Yuji|
|
抄録 |
<p>We applied an in situ polymerase chain reaction (PCR) hybridization method in order to detect human T-lymphotropic virus type I-infected cells in routinely-processed paraffin sections of the lung from 13 autopsied patients with adult T-cell leukemia (ATL). Previously reported protocol resulted in somewhat non-specific staining in our sections. Therefore, we used a hot start PCR method using specialized commercially-available polymerase in order to increase the specificity. Of 6 patients with ATL cell invasion into the lungs, 4 exhibited strong positive staining of almost all invading ATL cells. In contrast, 7 patients without ATL cell invasion into the lungs did not demonstrate any significant reactivity. Since the method described here is a relatively simple hot start method and does not yield false-positives, it may allow us to determine whether human T-lymphotropic virus type I (HTLV-I) associated disorders are related to lymphocytes integrating the HTLV-I genome.</p>
|
キーワード |
polymerase chain reaction
in situ hybridization
human T-lymphotopic virus type I
paraffin section
lung
|
Amo Type |
Article
|
発行日 |
1999-02
|
出版物タイトル |
Acta Medica Okayama
|
巻 |
53巻
|
号 |
1号
|
出版者 |
Okayama University Medical School
|
開始ページ |
1
|
終了ページ |
4
|
ISSN |
0386-300X
|
NCID |
AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
English
|
論文のバージョン |
publisher
|
査読 |
有り
|
PubMed ID |
10096731
|
Web of Sience KeyUT |
000078897700001
|