start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue=1 article-no= start-page=1 end-page=4 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=199902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Application of an in Situ PCR hybridization method to detection of human T-lymphotropic virus type I-infected cells in the lung. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We applied an in situ polymerase chain reaction (PCR) hybridization method in order to detect human T-lymphotropic virus type I-infected cells in routinely-processed paraffin sections of the lung from 13 autopsied patients with adult T-cell leukemia (ATL). Previously reported protocol resulted in somewhat non-specific staining in our sections. Therefore, we used a hot start PCR method using specialized commercially-available polymerase in order to increase the specificity. Of 6 patients with ATL cell invasion into the lungs, 4 exhibited strong positive staining of almost all invading ATL cells. In contrast, 7 patients without ATL cell invasion into the lungs did not demonstrate any significant reactivity. Since the method described here is a relatively simple hot start method and does not yield false-positives, it may allow us to determine whether human T-lymphotropic virus type I (HTLV-I) associated disorders are related to lymphocytes integrating the HTLV-I genome.

en-copyright= kn-copyright= en-aut-name=QiuYue en-aut-sei=Qiu en-aut-mei=Yue kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakeuchiTamotsu en-aut-sei=Takeuchi en-aut-mei=Tamotsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ChenBing-Kun en-aut-sei=Chen en-aut-mei=Bing-Kun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OhtsukiYuji en-aut-sei=Ohtsuki en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Kochi Medical School affil-num=2 en-affil= kn-affil=Kochi Medical School affil-num=3 en-affil= kn-affil=Kochi Medical School affil-num=4 en-affil= kn-affil=Kochi Medical School en-keyword=polymerase chain reaction kn-keyword=polymerase chain reaction en-keyword=in situ hybridization kn-keyword=in situ hybridization en-keyword=human T-lymphotopic virus type I kn-keyword=human T-lymphotopic virus type I en-keyword=paraffin section kn-keyword=paraffin section en-keyword=lung kn-keyword=lung END