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journal.pone.0033800.pdf 2.34 MB
Tani, Akio Institute of Plant Science and Resources, Okayama University
Takai, Yuichiro Research Institute of Environment, Agriculture and Fisheries, Osaka Prefectural Government
Suzukawa, Ikko Meiho-Construction
Akita, Motomu Faculty of Biological Engineering, Kinki University
Murase, Haruhiko Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University
Kimbara, Kazuhide Institute of Plant Science and Resources, Okayama University
Bryophytes, or mosses, are considered the most maintenance-free materials for roof greening. Racomitrium species are most often used due to their high tolerance to desiccation. Because they grow slowly, a technology for forcing their growth is desired. We succeeded in the efficient production of R. japonicum in liquid culture. The structure of the microbial community is crucial to stabilize the culture. A culture-independent technique revealed that the cultures contain methylotrophic bacteria. Using yeast cells that fluoresce in the presence of methanol, methanol emission from the moss was confirmed, suggesting that it is an important carbon and energy source for the bacteria. We isolated Methylobacterium species from the liquid culture and studied their characteristics. The isolates were able to strongly promote the growth of some mosses including R. japonicum and seed plants, but the plant-microbe combination was important, since growth promotion was not uniform across species. One of the isolates, strain 22A, was cultivated with R. japonicum in liquid culture and in a field experiment, resulting in strong growth promotion. Mutualistic symbiosis can thus be utilized for industrial moss production.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PUBLIC LIBRARY SCIENCE
© 2012 Tani et al.
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