JaLCDOI 10.18926/AMO/32873
フルテキストURL fulltext.pdf
著者 Matsuoka, Hiroyuki| Thuan, Dang Thi Vinh| van Thien, Huynh| Kanbe, Toshio| Jalloh, Amadu| Hirai, Makoto| Arai, Meiji| Dung, Nguyen The| Kawamoto, Fumihiko|
抄録 We conducted a survey for glucose-6-phosphate dehydrogenase (G6PD) deficiency using blood samples from male outpatients of a local hospital in southern Vietnam. Most of the samples were from the Kinh (88.9%), the largest ethnic group in Vietnam, with a small number (11.1%) coming from the K'Ho, Chauma, Nung, and Tay minorities. We detected 25 G6PD-deficient cases among 1,104 samples (2.3%), and read the open reading frame of G6PD. A novel mutation (352T>C) predicting an aminoacid change of 118Tyr>His was found in a 1-year-old Kinh boy. His G6PD activity was estimated to be less than 10% residual activity, although he did not show chronic hemolytic anemia. Thus, we categorized this variant as Class II and named it G6PD Bao Loc. In the Kinh population, G6PD Viangchan (871G>A, 1311C>T, intron 11 nt93T>C), one of the most common variants in continental Southeast Asian populations, was the highest (6/19), followed by variants originating from the Chinese such as G6PD Canton (1376G>T) (5/19), G6PD Kaiping (1388G>A) (3/19), G6PD Gaohe (95A>G) (1/19), and G6PD Quing Yuan (392G>T) (1/19). In addition, G6PD Union (1360C>T) (2/19), which originated from the Oceania, was also detected. These findings suggest that the Kinh people are derived from various ancestries from continental Southeast Asia, China, and Oceania. In contrast, all of the 5 deficient cases in the K'Ho population were G6PD Viangchan, suggesting that they were very close to Southeast Asian populations such as the Khmer in Cambodia and the Lao in Laos. It is interesting that G6PD Mahidol (487G>A), another common variant in continental Southeast Asian populations in Myanmar, Thailand, and Malaysia, has not been detected from the Vietnamese.
キーワード Bao Loc glucose-6-phosphate dehydrogenase defi ciency Kinh malaria Vietnam
Amo Type Original Article
発行日 2007-08
出版物タイトル Acta Medica Okayama
61巻
4号
出版者 Okayama University Medical School
開始ページ 213
終了ページ 219
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 17726510
Web of Science KeyUT 000248957100005
JaLCDOI 10.18926/AMO/30966
フルテキストURL fulltext.pdf
著者 Wang, Jichun| Luo, Enjie| Hirai, Makoto| Arai, Meiji| Abdul Manand, Encik Abdul Salim| Isa, Zaleha Mohamed| Hidayah, Noor Ishak| Matsuoka, Hiroyuki|
抄録 <p>The Malaysian people consist of several ethnic groups including the Malay, the Chinese, the Indian and the Orang Asli (aboriginal Malaysians). We collected blood samples from outpatients of 2 hospitals in the State of Selangor and identified 27 glucose-6-phosphate dehydrogenase (G6PD)-deficient subjects among these ethnic groups. In the Malay, G6PD Viangchan (871G&#65310;A, 1311C&#65310;T, IVS11 nt93T&#65310;C) and G6PD Mahidol (487G&#65310;A) types, which are common in Cambodia and Myanmar, respectively, were detected. The Malay also had both subtypes of G6PD Mediterranean:the Mediterranean subtype (563C&#65310;T, 1311C&#65310;T, IVS11 nt93T&#65310;C) and the Indo-Pakistan subtype (563C&#65310;T, 1311C, IVS11 nt93T). In Malaysians of Chinese background, G6PD Kaiping (1388G&#65310;A), G6PD Canton (1376G&#65310;T) and G6PD Gaohe (95A&#65310;G), which are common in China, were detected. Indian Malaysians possessed G6PD Mediterranean (Indo-Pakistan subtype) and G6PD Namoru (208T&#65310;C), a few cases of which had been reported in Vanuatu and many in India. Our findings indicate that G6PD Namoru occurs in India and flows to Malaysia up to Vanuatu. We also discovered 5 G6PD-deficient cases with 2 nucleotide substitutions of 1311C&#65310;T and IVS11 nt93T&#65310;C, but without amino-acid substitution in the G6PD molecule. These results indicate that the Malaysian people have incorporated many ancestors in terms of G6PD variants.</p>
キーワード Aborigine Chinese glucose-6-phosphate dehydrogenase Indian Malay
Amo Type Original Article
発行日 2008-10
出版物タイトル Acta Medica Okayama
62巻
5号
出版者 Okayama University Medical School
開始ページ 327
終了ページ 332
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 18985093
Web of Science KeyUT 000260391300007
JaLCDOI 10.18926/AMO/30730
フルテキストURL fulltext.pdf
著者 Arai, Meiji| Kosuge, Kazuko| Kawamoto, Fumihiko| Matasuoka, Hiroyuki|
抄録 <p>Deficiency of glucose-6-phosphate dehydrogenase (G6PD) causes acute hemolytic anemia triggered by oxidative drugs such as primaquine. It is therefore essential in malaria-endemic areas for malaria patients to be confirmed for their G6PD activity before taking primaquine. The WST-8 method, a newly established screening method for G6PD deficiency, has been demonstrated to be suitable for field conditions, particularly for on-site malaria surveys. Here we report a laboratory evaluation by this method of the reactivity of blood-spotted filters. A time-course experiment was conducted to evaluate the reactivity of blood samples spotted onto 4 types of filter paper, Whatman 31ET Chr (ET), 3MM Chr (3MM), P81, and Advantec No. 2 (AD2). The rank of the relative reaction intensity was ET &#62; 3MM = AD2 &#62; P81. Blood-spotted filters stored at 4 degrees centigrade gradually decreased G6PD reactivity with the passage of storage time, whereas those stored at room temperature rapidly reduced their reactivity. Unexpectedly, saponin supplementation reduced the reactivity of blood-spotted filters. In conclusion, 1) ET is the most suitable filter for the WST-8 method ; 2) blood-spotted filters stored in cold condition can be assayed within 14 days, or those stored at room temperature should be tested within 3 days ; and 3) reaction mixtures should not contain saponin.</p>
キーワード glucose-6-phosphate dehydrogenase G6PD deficiency blood-spotted filter WST-8 method malaria
Amo Type Article
発行日 2006-04
出版物タイトル Acta Medica Okayama
60巻
2号
出版者 Okayama University Medical School
開始ページ 127
終了ページ 134
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 16680190
Web of Science KeyUT 000237001900009