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ID 17220
Eprint ID
17220
フルテキストURL
Thumnail 90_159.pdf 361 KB
タイトル(別表記)
Free amino acids and ammonia in rat blood and cerebral cortex following L-asparaginase administration
著者
庄盛 敏廉 岡大医学部脳代謝研究施設病態生化学部門脳代謝神経科
金行 孝雄 岡大医学部脳代謝研究施設病態生化学部門脳代謝神経科
土井 亨 岡大医学部脳代謝研究施設病態生化学部門脳代謝神経科
三谷 和史 岡大医学部脳代謝研究施設病態生化学部門脳代謝神経科
高坂 睦年 岡大医学部脳代謝研究施設病態生化学部門脳代謝神経科
抄録
The enzyme L-asparaginase, which catalyzes the hydrolysis of the amino acids L-asparagine and L-glutamine to aspartic acid, glutamic acid and ammonia, is useful in the treatment of patients with acute leukemia. However, acute and delayed cerebral dysfunction often occurs in leukemic patients treated by L-asparaginase. Large amounts of L-aspartic acid, L-glutamic acid and ammonia may be liberated by the enzymatic action of L-asparaginase on its substrates L-asparagine and L-glutamine. The ensuing amino acid depletion or its excess may affect brain metabolism and lead to clinical abnormalities. The present paper reports on free amino acid levels and ammonia content in rat serum and cerebral cortex after L-asparaginase administration. Wistar male rats were used throughout this investigation. The acute experimental animals were administered 0.2ml of normal saline containing L-asparaginase (about 500 I.U./Kg B.W.) intraperitoneally and the acute control animals were given 0.2ml of normal saline intraperitoneally. 24h after this single administration, all rats were decapitated, and blood and cortex were collected for the estimation of free amino acids and ammonia. The acute L-asparaginase group showed some significant differences in serum compared to the control group. The most remarkable finding was the absence of asparagine. Aspartic acid and ammonia were elevated significantly; valine and methionine decreased significantly. In the cerebral cortex, aspartic acid and ammonia showed an insignificant change, but some other amino acids serine, glutamine, glycine, GABA and l-methylhistidine were significantly elevated. The chronic experimental group received 0.2ml of saline containing L-asparaginase (about 500 I.U./Kg B.W.) intraperitoneally once a day for 7 consecutive days. The chronic control group received 0.2ml of saline intraperitoneally in the same regime with the experimental group. All rats were fed a ground commercial diet and water ad libitum. The animals were housed in two large cages (the control cage and the experimental cage) in a dark-light room with alernating 12h dark-light cycles. Twenty-four hours after the last administration, all rats were decapitated for collection of blood and cerebral cortex. The chronic L-asparaginase group showed the absence of asparagine and significant increase of aspartic acid in serum similar to the acute L-asparaginase group but an insignificant increase of ammonia in blood. In addition to this finding, threonine, serine, glutamic acid and glycine were elevated significantly. In the cerebral cortex of the chronic L-asparaginase-treated animals, threonine, glycine and l-methylhistidine were significantly elevated compared to the control. It will be difficult to infer from our present data that the cerebral dysfunction induced by L-asparaginase may be correlated mainly with increased levels of aspartic acid, glutamic acid and ammonia in the cerebral cortex.
発行日
1978-02-28
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
90巻
1-2号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
159
終了ページ
165
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/90/1-2/90_1-2_159/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma