岡山医学会 Acta Medica Okayama 0030-1558 71 6-1 1959 高温高湿環境のB(1)代謝に及ぼす影響に関する研究 第I編 高温高湿環境下B(1)欠乏食飼育シロネズミの組織B(1)量 2885 2894 EN Masamichi Hohgaku With the purpose to determine the influences of hot and highly humid environment on the B(1)-metabolism the author pursued the changes in the tissue B(1)-eontent and body weight in albino rats fed various foods for 30 days under a fixed hot environment and obtained the following results. 1. The concentration and quantity of the tissue B1 in the albino raised with common foods under hot environment decreased temporarily 5 days after the start of experiment, but thereafter with the lapse of time the B(1)-concentration gia ally re ed to the normal level. However, the absolute B1-content in the liver alone remained at a low level even 30 days later. 2. When young albino rats were raised with Shimazono B(1)-deficient diet or with low fat Shimazono B(1)-deficient diet for 30 days under hot environment, the decrease in the tissue B(1)-concentration showed no significant difference from that in the control group raised under the normal environment. Howeyer, the B(1)-content in the liver of all those raised under the hot environment alone showed a lower value than in the control group. 3. The tissue B(1)-concentration and quantity in the young albino rats raised with the low fat diet under the hot environment showed markedly lower values than those observed in the group raised with the B1-deficient Shimazono diet. 4. The gain in the body weight of the albino rats raised with various foods under hot environment was markedly poorer than that in the control group raised under the normal environments. However, the loss in the body weight of the young albino rats fed B(1)-deficient diet was less in the group raised under the hot environment than that in the control raised under the normal temperature. 5. The fluctuations in the B(1)-content and concentration of the brain were remarkably small throughout the experiment in both environmental conditions when compared with those of other tissues. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 71 6-1 1959 高温高湿環境のB(1)代謝に及ぼす影響に関する研究 第II編 高温高湿環境下負荷B(1)の組織移行及び尿中B(1)排泄量 2895 2901 EN Masamichi Hohgaku In order to see the utilization of B(1)-intake under a hot and highly humid environment, the author raised albino rats under a hot and highly humid environment keeping the hygrometric temperature around 28-30°C and administering B(1), stimated the tissue B(1)-content, changes in the body weignt, the amount of food consumed, and the B(1)-content excreted in the urine. The following are the results. 1. When the daily dose of 6γ B(1) was adminstered for one week to albino rats raised with B1-deficient Shimazono diet under a hot environment. the B(1)-concentration of the heart was 1.3 times that of the control raised under the normal environment, and the B(1)-concentration in the liver, kidneys, and brain was likewise somewhat higher but the B(1)-content was smaller than that in the control. 2. When the daily dose of 40γ B(1) was given one week to the albino rats raised with the B(1)-defieient Shimazono diet, the tissne B(1)-concentration was rather higher in the heart and kidneys than in the control, but the B(1)-content was lower in the liver and no differences could be observed in other tissues as compared with the control. 3. After injectiog 0.5r/g B(1) to the albino rats raised with B1-deficient diet under the hot environment and by bathing in hot water for 10 minutes every one honr the concentration of tissne B(1) after 9 hours showed no difference from that in the control, but the B(1)-content was generally lower, especially the B(1)-content in the heart and liver was only about 60-70 per cent of the control. 4. The B(1)-content excreted in the urine of the albino rats raised uuder a hot environment in the case of daily administration of B(1) under the dosc of 6γ showed no difference from that in the control raised under the normal temperature, but when the daily dos of B(1) was raised to 40γ or 80γ, the B(1)-content excreted in the urine at last rose to 1.3 times that on the control. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 71 6-1 1959 高温高湿環境のB(1)代謝に及ぼす影響に関する研究 第III編 高温高湿環境下飼育シロネズミの尿中ピラミン排泄量 2903 2909 EN Masamichi Hohgaku By estimating the contents of pyramin and vitamin B(1) excreted in the urine of the albino rats raised under the hot and highly humid environment of hygrometric temperature at 28-30°C, the author obtained the following results. 1. When the daily dose of 40γ B(1) was administered to the albino rats being raised under a hot environment, the mean averago of the pyramin content excreted in the urine per day by an individual rat was around 3.52-3.38γ, showing no difference from that of the control. While the B(1)-quantity excreted in the ruine per day was around 6.28-5.8γ, proving to be 1.3 times that of the control. In this instance, the quantity per 1,000 calories of non-fatty substance taken in by B(1) remaining in the body amounted to about 0.68-0.64 mg, proving greater than that in the control (0.45-0.47 mg). 2. When B(1) was administered in various under the hot environment, the pyramin content excreted in the urine increased in proportion to the increasing dose of B(1), but the degree of the increase, being only slight, differed not much from that of the control. The amount of B(1) excreted in the urine, when the B1 dose was increased to 40γ or 80γ, was 6.4γ and 26.1γ respectively, each value proving to be 1.3 times that of the control (4.4γ and 21.3γ respectively). 3. When the common foods was given freely under the hot environment, the B1-content and pyramin-content excreted in the urine were 4.9-5.8γ and 3.8-3.9γ respectively, both being less than the respective values in the control (6.2-7.2γ and 4.7-4.2γ). This seems to be due to the decrease in the food intake with the attendant decrease in the amount of B(1)-intake. 4. The gain in the body weight and the amount of food intake in the case of the albino rats under the hot and highly humid environment were for poorer than those of the control raised under the normal environment. No potential conflict of interest relevant to this article was reported.