フルテキストURL JRNC_313_1_169.pdf
著者 Kudo, Ken-ichi| Hanafusa, Tadashi| Ono, Toshiro|
抄録 Monoterpenes are naturally occurring hydrocarbons composed of two units of isoprenes. They exhibit antioxidant activity to scavenge reactive oxygen species, such as hydroxyl radicals. We investigated the potential of monoterpenes such as thymol, linalool, and menthol to act as radioprotectants. The proliferation of EL4 cells, a mouse lymphoma cell line, treated with linalool at a concentration of 500 μM or more was not affected by X-ray irradiation. Plasmid-nicking assay performed using formamidopyrimidine-DNA glycosylase showed that linalool prevented single strand breaks and oxidized purines on pUC19 plasmid DNA. These findings indicate that linalool has the ability to scavenge reactive oxygen species and is a potential radioprotector.
キーワード Monoterpenes Linalool X-ray irradiation Reactive oxygen species SSB
備考 The final publication is available at Springer
発行日 2017-07
出版物タイトル Journal of Radioanalytical and Nuclear Chemistry
313巻
1号
出版者 Springer
開始ページ 169
終了ページ 174
ISSN 0236-5731
NCID AA11120189
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
論文のバージョン author
関連URL https://doi.org/10.1007/s10967-017-5268-0
著者 Nishiyama, Yuichi| Hanafusa, Tadashi| Yamashita, Jun| Yamamoto, Yoko| Ono, Toshiro|
発行日 2015-06-20
出版物タイトル Journal of Radioanalytical and Nuclear Chemistry
資料タイプ 学術雑誌論文
著者 Hanafusa, Tadashi| Mohamed, Ali Eldib Ali| Kitaoka, Kenta| Ohue, Yoshihiro| Nakayama, Eiichi| Ono, Toshiro|
発行日 2011-02-01
出版物タイトル Cancer Letters
301巻
1号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/32656
フルテキストURL fulltext.pdf
著者 Hada, Hajime| Koide, Norio| Hanafusa, Tadashi| Sakaguchi, Kosaku| Shinji, Toshiyuki| Sasaki, Shunsuke| Oka, Takahiko| Takayama, Niro| Yumoto, Yasuhiro| Tsuji, Takao|
抄録 <p>We detected an antibody to HCV envelope protein (E1) in sera of patients with HCV-related chronic liver diseases (20 patients with chronic hepatitis and 5 patients with liver cirrhosis) by Western blotting using the fusion protein of E1 envelope protein and beta-galactosidase as an antigen. The antibody to HCV E1 (anti-HCV E1) was detected in 8 (42%) of 19 patients positive for HCV-RNA (16 were positive and 3 were negative for antibody to C100-3) and in 1 (17%) of 6 patients negative for HCV-RNA but positive for antibody to C100-3. HCV-RNA was detected in 8 (89%) of 9 anti-HCV E1 positive sera. The value of alanine aminotransferase was significantly higher in patients positive for anti-HCV E1 than in patients negative for the antibody. Although an antibody to the envelope protein of HCV is suspected to be one of the candidates of virus-neutralizing antibodies, our results suggest this hypothesis appears to be unlikely.</p>
キーワード hepatitis C virus envelope antibody Western blotting
Amo Type Article
発行日 1992-10
出版物タイトル Acta Medica Okayama
46巻
5号
出版者 Okayama University Medical School
開始ページ 367
終了ページ 370
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1279946
Web of Sience KeyUT A1992JX49500007
JaLCDOI 10.18926/AMO/32196
フルテキストURL fulltext.pdf
著者 Hada, Hajime| Koide, Norio| Takabatake, Hiroyuki| Hanafusa, Tadashi| Tsuji, Takao|
抄録 It has been reported that the envelope region located at the 3' portion of the structural protein coding region is one of the most variable regions at both nucleotide and amino acid sequence levels in the hepatitis C virus (HCV) genome. We cloned HCV cDNA fragments of an envelope protein coding region (HCVNK), which were derived from serum of a Japanese patient with hepatocellular carcinoma and were amplified by polymerase chain reaction. After determining the nucleotide sequence, deduced amino acid sequence of the envelope protein region was compared with those of six HCV strains already published (HCJ1, HCVUS, HCJ4, HCVJH, HCVJ and HCVBK). Homology analysis among the strains revealed that the seven strains were classified into two subtypes; a US subtype (HCJ1 and HCVUS) and a Japanese subtype (HCJ4, HCVJH, HCVJ, HCVBK and HCVNK), since percentage homologies between two subtypes (70.3-77.3%) were significantly lower than those within each subtype (83.9-93.5%). Detailed analysis of the amino acid sequences also indicates that the region at aa246-aa258, tentatively named intersubtype variable region-1, may distinguish the US subtype from the Japanese subtype.
キーワード hapatitis C virus envelope DNA sequecing homology intersubtype variable region
Amo Type Article
発行日 1991-10
出版物タイトル Acta Medica Okayama
45巻
5号
出版者 Okayama University Medical School
開始ページ 347
終了ページ 355
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1661558
Web of Sience KeyUT A1991GN53800009
著者 Hanafusa, Tadashi| Shinji, Toshiyuki| Shiraha, Hidenori| Nouso, Kazuhiro| Iwasaki, Yoshiaki| Yumoto, Eichiro| Ono, Toshiro| Koide, Norio|
発行日 2005-01-20
出版物タイトル BMC Cancer
5巻
資料タイプ 学術雑誌論文
著者 永松 知洋| 花房 直志| 金野 郁雄| 迫田 晃弘| 花元 克巳| 山岡 聖典| 小野 俊朗|
発行日 2008-12
出版物タイトル 環境制御
30巻
資料タイプ 学術雑誌論文
著者 花房 直志|
発行日 1989-03-28
出版物タイトル
資料タイプ 学位論文