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ID 42575
フルテキストURL
Thumnail 69_1527.pdf 307 KB
タイトル(別表記)
Studies on the Influence of Buffer on the Bacterial Respiratiration
著者
矢部 芳郎 岡山大学医学部微生物学教室
秋田 悦示 岡山大学医学部微生物学教室
秋田 和男 岡山大学医学部微生物学教室
抄録
The preparation of resting cells is one of the most important and most fundamental procedures for the physiological studies on bacteria. In order to know how we should do to get the resting cells suitable to the purposes of experiments, the author studied the influence of washing and suspending on the respiratory activity of bacteria. The results are summarized as follows: 1) For the purpose to obtain the resting cells of high respiratory activity, washing with phosphate buffer is better than that with distilied water or physiological saline solution. 2) When the prepared resting cells are used in a short time after the preparation, the respiratory activity is the same regardless of the sorts of the suspending solutions, distilled water, saline solution and phosphate buffer. 3) Borate and phthalate buffers of high concentration somewhat inhibit the respiration. However, in order to make the shift of pH less, M/15 (final concentration) is good. Phosphate buffer, even of M/15, does not inhibit the respiration. 4) In borate or phthalate buffers, the fall of respiratory activity is diminished by the addition of small amount of phospate.
発行日
1957-06-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
69巻
6号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
1527
終了ページ
1531
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/69/6/69_6_1527/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/
言語
Japanese
著作権者
Copyright© 岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma