JaLCDOI 10.18926/AMO/45270
フルテキストURL 65_2_113.pdf
著者 Morikawa, Toshio| Yamamoto, Yuji| Miyaishi, Satoru|
抄録 We have developed a new method for sex determination based on simultaneous detection of the SRY (sex-determining region Y), STS (steroid sulfatase) and amelogenin (AMELX and AMELY) gene regions and their homologous sequences. The sex of 246 blood samples was correctly determined by this method. An AMELY-deleted male sample, which would have been erroneously considered female based solely on analysis of the amelogenin locus, was successfully identified as male by the present method. The detection limit of this method was 63 pg of genomic DNA, and the male DNA component could be detected from mixed samples having a male:female ratio as low as 1:10. This method was useful for degraded DNA and possessed the human specificity. Practical application to 35 autopsy cases is described.
キーワード sex determination SRY (sex-determining region Y) multiplex PCR forensic casework
Amo Type Original Article
発行日 2011-04
出版物タイトル Acta Medica Okayama
65巻
2号
出版者 Okayama University Medical School
開始ページ 113
終了ページ 122
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2011 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 21519369
Web of Sience KeyUT 000289818800007
JaLCDOI 10.18926/AMO/31726
フルテキストURL fulltext.pdf
著者 Yoshitome, Kei| Ishikawa, Takaki| Inagaki, Sachiyo| Yamamoto, Yuji| Miyaishi, Satoru| Ishizu, Hideo|
抄録 <p>We encountered a rare case of suffocation by an advertising balloon filled with pure helium gas. Suffocation caused by inhalation of atmosphere lacking in oxygen is not exceptional, but reports of death by suffocation due to a pure inert gas such as helium are very rare. In this case, the balloon mooring on the ground was enclosed, warning signs were displayed, and it was clear that entering the balloon filled with an atmosphere lacking in oxygen was extremely dangerous and should not be done; the accident did, however, occur. Accidents of this kind may occur in the future unless appropriate education and countermeasures are taken.</p>
キーワード asphyxia suffocation helium advertising balloon atmosphere lacking in oxygen
Amo Type Article
発行日 2002-02
出版物タイトル Acta Medica Okayama
56巻
1号
出版者 Okayama University Medical School
開始ページ 53
終了ページ 55
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 11873946
Web of Sience KeyUT 000174031300010
JaLCDOI 10.18926/AMO/31971
フルテキストURL fulltext.pdf
著者 Imabayashi, Kiyomi| Yamamoto, Yuji| Inagaki, Sachiyo| Doi, Yusuke| Yoshitome, Kei| Miyaishi, Satoru| Ishizu, Hideo|
抄録 <p>We have improved on conventional methods for HLA-DRB1 genotyping and devised a new method that is simple, cost-effective, and adequately applicable to routine forensic practice. This method consists of group-specific polymerase chain reaction (PCR) of the exon 2 region of the HLA-DRB1 gene and simultaneous detection of single nucleotide polymorphisms (SNPs) at multiple sites using multiplex primer extension reactions. With this method, we successfully detected HLA-DRB1 genotypes from the following materials: the peripheral blood of 142 donors, 6 aged saliva stains of known DRB1 genotype stored for 5-10 years at room temperature, 10 aged bloodstains of unknown DRB1 genotype stored for 29 years at room temperature, and minimal bloodstains and saliva stains from 3 donors of known DRB1 genotypes. Furthermore, we were able to type DRB1 alleles of the minor component in mixed samples at a proportion of 1/1,000 or 1/10,000. In a criminal case, DRB1 alleles detected from mixed bloodstains on a sword found at the scene enabled us to explain the case. This method is expected to be useful for forensic medicine.</p>
キーワード HLA-DRB1 genotyping group specific primer single nucleotide polymorphism multiplex primer extension reactions application to mixed samples
Amo Type Article
発行日 2005-10
出版物タイトル Acta Medica Okayama
59巻
5号
出版者 Okayama University Medical School
開始ページ 179
終了ページ 194
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 16286957
Web of Sience KeyUT 000232835600002
JaLCDOI 10.18926/AMO/32819
フルテキストURL fulltext.pdf
著者 Okamoto, Osamu| Yamamoto, Yuji| Inagaki, Sachiyo| Yoshitome, Kei| ishikawa, Takaki| Imabayashi, Kiyomi| Miyaishi, Satoru| Ishizu, Hideo|
抄録 <p>Allele and genotype frequencies for 15 short tandem repeat (STR) polymorphisms--D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX and FGA--in a Japanese population were estimated. No deviations of the observed allele frequency from Hardy-Weinberg equilibrium expectations were found for any of the systems studied. Between 2 new pentanucleotide STR loci, Penta E and Penta D, for which there is only limited data regarding the allelic distribution in Japanese, the Penta E locus was found to be highly polymorphic and exhibited a tri- or tetra-modal distribution pattern having allelic peaks with 5, 11, 15 and 20 repeats. The distribution was significantly different from that of the other ethnic groups. Statistical parameters of forensic importance, the power of discrimination (PD), observed and expected heterozygosity values (H), polymorphism information content (PIC), power of discrimination (PD), matching probability (pM), power of exclusion (PE), and typical paternity index (PI), were calculated for the loci. These parameters indicated the usefulness of the loci in forensic personal identification and paternity testing among Japanese. The systems Penta E, FGA, D18S51 and D8S1179 were the most informative. This method was successfully applied to forensic personal identification and paternity testing among Japanese, thereby confirming its efficacy for forensic practice.</p>
キーワード population data DNA typing short tandem repests personal identification paternity testing
Amo Type Article
発行日 2003-04
出版物タイトル Acta Medica Okayama
57巻
2号
出版者 Okayama University Medical School
開始ページ 59
終了ページ 71
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 12866745
Web of Sience KeyUT 000182520400003
JaLCDOI 10.18926/AMO/31707
フルテキストURL fulltext.pdf
著者 Shigeta, Yoshiaki| Yamamoto, Yuji| Doi, Yusuke| Miyaishi, Satoru| Ishizu, Hideo|
抄録 <p>We describe a modified method for typing a polymorphic microsatellite D12S391 locus by PCR using a newly designed primer pair. This primer pair produces shorter D12S391 amplified fragments (104-156 bp) than the primer pair originally described by Lareu et al. (209-261 bp). The detection system for the D12S391 locus using the new primer pair and capillary electrophoresis (CE) analysis was evaluated using various forensic samples. The typing results from 70 DNA samples using the new primer pair and the original primer pair were completely identical. One hundred twenty-five amplified fragments from D12S391 alleles were sized correctly within +/- 0.25 bp of the D12S391 allelic ladder. A rare allele, 19.3, previously found only in Caucasians, was found for the first time in a Japanese subject, and it was clearly distinguished from allele 20 by the CE analysis. This detection system was sensitive and could detect D12S391 types from 16 pg of genomic DNA, and from a minor component at a ratio of 1:10 in mixed samples. This system was more useful for the analysis of degraded DNA than was the method using the original primer pair, and could detect D12S391 types from bloodstains that had been stored for 26 years. In addition, the specificity of the method was demonstrated using nonhuman DNA.</p>
キーワード short tandem repeats D12S391 forensic application capillary electrophoresis
Amo Type Article
発行日 2002-10
出版物タイトル Acta Medica Okayama
56巻
5号
出版者 Okayama University Medical School
開始ページ 229
終了ページ 236
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 12530506
Web of Sience KeyUT 000178668100003
JaLCDOI 10.18926/AMO/31815
フルテキストURL fulltext.pdf
著者 Kamizato, Eigo| Yoshitome, Kei| Yamamoto, Yuji| Iwase, Toshihide| Tsuda, Toshihide| Miyaishi, Satoru| Doi, Hiroyuki|
抄録 <p>The annual number of suicides in Japan increased sharply in 1998, and since that time it has consistently exceeded 30,000 per year. In this study, we analyze a database of personal and background characteristics of 824 cases (605 men, 219 women) who completed suicide in Okayama Prefecture in 2002 and 2003. The data were obtained with cooperation from the police. Using the methodologies in a previous European study as a model, we classified the suicide methods into 8 categories. To examine the generational and regional differences in the choice of methods, we stratified the sample into 4 age groups (&#60;-24, 2544, 4564, and &#62;-65) and 2 regional groups (Okayama/Kurashiki vs. other areas). Our results on gender differences in 7 of the suicide methods were mostly similar to the European data. However, our data showed a remarkably higher proportionate male-to-female mortality ratio for poisoning by other substances (ICD-10, X65-X69 codes) (1.83, 1.15-2.92). In terms of generational differences in the choice of suicide methods, the Mantel-Haenszel test of homogeneity was significant for most of the categories in our study, suggesting an impact of age on how people commit suicide. There were no remarkable regional differences in our sample. An epidemic curve for suicides via carbon monoxide poisoning using charcoal briquets revealed a trend of time clustering not observed in the other 6 means. The database constructed and used in this study contains richer information than conventional death statistics and is expected to provide helpful knowledge and insights for future epidemiological studies.</p>
キーワード suicide methods gender-specific legal medicine cluster suicide
Amo Type Original Article
発行日 2009-08
出版物タイトル Acta Medica Okayama
63巻
4号
出版者 Okayama University Medical School
開始ページ 177
終了ページ 186
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 19727202
Web of Sience KeyUT 000269228400003
JaLCDOI 10.18926/AMO/31301
フルテキストURL fulltext.pdf
著者 Yano, Akemi| Yamamoto, Yuji| Miyaishi, Satoru| Ishizu, Hideo|
抄録 <p>We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp2 = 0.723 and Hp1s = 0.277. Genotyping of Hp was possible with 0.3 ng of DNA and with 0.125 microliter of blood. It was also possible with whole blood left at room temperature for a month and also with the bloodstains left at room temperature for three years. In the heated blood samples, both alleles, Hp2 and Hp1s, were detected in those heated at 100 degrees C for 2 h. In bloodstains, Hp2 and Hp1s were detected in samples heated at 100 degrees C for 2 h and 120 degrees C for 30 min. In addition, the genotype could be detected in samples other than blood such as saliva, hair roots, tissue sections and dental pulps. The present method for Hp genotyping is expected to become a useful method in forensic analysis.</p>
キーワード DNA polymorphism haptoglobin polymerase chain reaction allele-specific amplification personal identification
Amo Type Article
発行日 1998-08
出版物タイトル Acta Medica Okayama
52巻
4号
出版者 Okayama University Medical School
開始ページ 173
終了ページ 181
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9781267
Web of Sience KeyUT 000075623600001
JaLCDOI 10.18926/AMO/32024
フルテキストURL fulltext.pdf
著者 Ono, Toshiaki| Miyaishi, Satoru| Yamamoto, Yuji| Yoshitome, Kei| Ishikawa, Takaki| Ishizu, Hideo|
抄録 <p>We developed a method for human identification of forensic biological materials by PCR-based detection of a human-specific sequence in exon 3 of the myoglobin gene. This human-specific DNA sequence was deduced from differences in the amino acid sequences of myoglobins between humans and other animal species. The new method enabled amplification of the target DNA fragment from 30 samples of human DNA, and the amplified sequences were identical with that already reported. Using this method, we were able to distinguish human samples from those of 21 kinds of animals: the crab-eating monkey, horse, cow, sheep, goat, pig, wild boar, dog, raccoon dog, cat, rabbit, guinea pig, hamster, rat, mouse, whale, chicken, pigeon, turtle, frog, and tuna. However, we were unable to distinguish between human and gorilla samples. This method enabled us to detect the target sequence from 25 pg of human DNA, and the target DNA fragment from blood stored at 37 degrees C for 6 months, and from bloodstains heated at 150 degrees C for 4 h or stored at room temperature for 26 years. Herein we also report a practical application of the method for human identification of a bone fragment.&#60;/P&#62;</p>
キーワード species identification myoglobin polymerase chain reaction
Amo Type Article
発行日 2001-06
出版物タイトル Acta Medica Okayama
55巻
3号
出版者 Okayama University Medical School
開始ページ 175
終了ページ 184
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 11434430
Web of Sience KeyUT 000169512600004
JaLCDOI 10.18926/AMO/30512
フルテキストURL fulltext.pdf
著者 Takata, Shingo| Yamamoto, Yuji| Ishizu, Hideo|
抄録 <p>A method of genotyping IgA2 alleles in the human immunoglobulin alpha 2 heavy chain constant region (C alpha 2 gene) was developed by using the polymerase chain reaction (PCR). By this method, the genotype was determined by discriminating base substitution in the 3'-flanking region of alleles, A2m*1 and A2m*2, which manifest A2m serum types, by nested PCR using allele-specific primers. Three types, IgA2*1/IgA2*1, IgA2*2/IgA2*1, and IgA2*2/IgA2*2, were detected from DNA extracted from lymphocytes. Genotyping was possible from 100 pg of DNA by this method. The estimated allele frequency in 318 Japanese subjects was 0.561 for IgA2*1 and 0.439 for IgA2*2. Analysis of 29 cases of paternity tests suggested that the data follow Mendel's law of inheritance. This genotype could also be detected in whole blood, blood stains, saliva stains, and various organs and tissues. These results suggest the usefulness of the present method for paternity testing and individual identification in forensic medicine.</p>
キーワード polymorphism deoxryibonucleic acid(DNA) immunoglobulin alpha 2 polymerase chain reaction(PCR) allele-specific amplificartion
Amo Type Article
発行日 1996-02
出版物タイトル Acta Medica Okayama
50巻
1号
出版者 Okayama University Medical School
開始ページ 1
終了ページ 9
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8701775
Web of Sience KeyUT A1996TY06000001
JaLCDOI 10.18926/AMO/31305
フルテキストURL fulltext.pdf
著者 Inoue, Seiichi| Yamamoto, Yuji| Okamoto, Osamu| Murakami, Hiroki| Miyaishi, Satoru| Isizu, Hideo|
抄録 <p>A sensitive method of HLA-DRB1 typing was devised using a semi-nested polymerase chain reaction (PCR) followed by a restriction fragment length polymorphism (RFLP) analysis (semi-nested PCR-RFLP method). The first-round amplification (30 cycles) of the semi-nested PCR was performed using DRB generic primer pairs and the second round of PCRs (20 cycles) were performed using DRB1 group-specific primers. The products of the second round PCRs were digested with restriction endonucleases for the typing of HLA-DRB1 alleles. By this method, HLA-DRB1 typing was possible from 10 pg of genomic DNA extracted from lymphocytes and from 0.5 microliter of 1,000 times diluted blood without DNA extraction. HLA-DRB1 alleles could be typed from a 2-mm long bloodstained cotton thread prepared from 10 times diluted blood and from a 2-mm thread of whole blood bloodstains stored at room temperature for 2 years. From the mixture of blood of two individuals with different genotypes, DRB1 alleles of the minor component were detected down to 1/1,000 of the major component. This semi-nested PCR-RFLP method is useful for HLA-DRB1 typing from extremely small amounts of DNA and from mixed samples.</p>
キーワード polymorphism HLA-DRB1 polymerase chain reaction dsmi-nested PCR restricton fragment length polymotphism
Amo Type Article
発行日 1998-12
出版物タイトル Acta Medica Okayama
52巻
6号
出版者 Okayama University Medical School
開始ページ 289
終了ページ 296
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9876765
Web of Sience KeyUT 000077707300002
JaLCDOI 10.18926/AMO/47011
フルテキストURL 65_5_299.pdf
著者 Itani, Miki| Yamamoto, Yuji| Doi, Yusuke| Miyaishi, Satoru|
抄録 Postmortem degradation of DNA was quantitatively estimated. Brain, liver, kidney and muscle samples were obtained from sacrificed rats left at 20℃ or 4℃. The quantity of DNA was measured by real-time PCR using a primer set for a sequence in the Rsrc 1 gene. When the quantity of amplified DNA using 10ng Human Genomic DNA was defined as 100 RFU, the quantities in the brain, liver, kidney and skeletal muscle (each 2μg of dry weight) on the day of sacrifice were 253±11, 338±22, 556±14 and 531±12 Relative Fluorescence Units (RFU), respectively (mean±S.E., n=5). The quantity of amplified DNA decreased to below 10 RFU in 1-3 weeks in the liver, kidney and skeletal muscle at 20℃, while that in the brain was more than 10 RFU for six weeks, demonstrating the usefulness of the brain as a sample for DNA analysis of decaying corpses. It was suggested that quantifying the amplified DNA in the brain at 20℃ and in the liver at 4℃ as well as the ratio of the quantity of amplified DNA in the liver to the brain at 4℃ might be useful for diagnosing time of death. This study provides the first quantitative analysis of the postmortem progress of DNA degradation in the corpse.
キーワード DNA degradation postmortem interval personal identification
Amo Type Original Article
発行日 2011-10
出版物タイトル Acta Medica Okayama
65巻
5号
出版者 Okayama University Medical School
開始ページ 299
終了ページ 306
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2011 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 22037266
Web of Sience KeyUT 000296116400003
JaLCDOI 10.18926/AMO/32816
フルテキストURL fulltext.pdf
著者 Ishikawa, Takaki| Miyaishi, Satoru| Tachibana, Toshiaki| Yamamoto, Yuji| Ishizu, Hideo|
抄録 <p>In this study we used paraffin-embedded human pituitary obtained from 248 autopsy cases and identified mixed cell follicles by the immunohistochemical method. We examined the number and size of the mixed cell follicles, and the ratio of each component cell of these follicles, in the anterior pituitary at various age groups. The number of follicles increased with age, and the size of the follicles also tended to enlarge with age. Statistical analysis showed that a high correlation existed between age and the number or the size of the mixed cell-follicles formed by various adenohypophyseal cells. In addition, when the proportions of the different cell types that formed the follicles were examined, sex differences were observed with aging for the GH cells, the PRL cells, and the gonadotroph (GTH) cells, while no changes were observed with aging in both men and women for the ACTH cells and TSH cells. These results indicate that the number, size, and ratio of each component cell of follicles in the anterior pituitary are adequately applicable for the purpose of age estimation in routine forensic medicine.</p>
キーワード mixed cell-follicle human anterior pituitary age estimation
Amo Type Article
発行日 2003-04
出版物タイトル Acta Medica Okayama
57巻
2号
出版者 Okayama University Medical School
開始ページ 83
終了ページ 89
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 12866748
Web of Sience KeyUT 000182520400006
著者 石津 日出雄| 宮石 智| 山本 雄二| 高田 真吾|
発行日 1993-10
出版物タイトル 岡山医学会雑誌
105巻
9-10号
資料タイプ 学術雑誌論文
著者 宮石 智| 北尾 孝司| 守屋 文夫| 山本 雄二| 石津 日出雄| 石井 啓行|
発行日 1991-10
出版物タイトル 岡山医学会雑誌
103巻
9-10号
資料タイプ 学術雑誌論文
著者 石川 隆紀| 宮石 智| 山本 雄二| 吉留 敬| 稲垣 幸代| 岡村 倫彦| 石津 日出雄|
発行日 2001-12-31
出版物タイトル 岡山医学会雑誌
113巻
3号
資料タイプ 学術雑誌論文
著者 石川 隆紀| 宮石 智| 土井 裕輔| 高田 智世| 今林 貴代美| 稲垣 幸代| 吉留 敬| 山本 雄二| 石津 日出雄|
発行日 2003-01-31
出版物タイトル 岡山医学会雑誌
114巻
3号
資料タイプ 学術雑誌論文
著者 山本 祐司|
発行日 1985-10-30
出版物タイトル 岡山医学会雑誌
97巻
9-10号
資料タイプ 学術雑誌論文